IFNAR1和IFNAR2在妊娠早期牛胎盘组织中的表达研究

发布时间：2018-04-23 05:41

本文选题：IFNAR1 + IFNAR2　；参考：《西北农林科技大学》2017年硕士论文

【摘要】：干扰素-τ(即IFNT)是一种I型干扰素,它是一种由妊娠滋养层分泌的抗黄体溶解因子。IFNT通过结合其受体IFNAR1和IFNAR2传递信号或者刺激某些因子的表达以建立母体妊娠识别信号。截止目前,有关IFNT受体(IFNAR1和IFNAR2)在奶牛胎盘组织上的表达定位及其作用的研究尚不够完善。本研究运用石蜡切片制作、HE及PAS染色技术对妊娠早期牛胎盘显微结构进行观察,以确定牛胎盘子叶及肉阜结构特征及发育特点,形成妊娠早期牛胎盘发育的组织结构图谱;进一步通过免疫荧光染色和qRT-PCR技术对妊娠早期牛胎盘组织中IFNAR1、IFNAR蛋白及其mRNA的细胞定位和表达规律进行研究,分析IFNAR1和IFNAR在妊娠早期牛胎盘发育中的功能意义。结果如下:1.妊娠早期的牛胎盘为子叶型/上皮绒毛膜胎盘,其子宫肉阜为半圆的穹顶状。随着孕龄增大,胎儿尿囊绒毛膜绒毛不断分枝、变长、并增大与母体子宫内膜上皮的嵌合,从而不断加大与母体胎盘区子宫内膜上皮的联系。妊娠27d后,胎儿绒毛膜上皮由单核细胞和PAS染色各异的三类滋养层双核巨细胞组成,并存在双核巨细胞迁移进入肉阜陷窝与子宫内膜上皮细胞融合而成的合胞体现象;妊娠50d,可观察到程度较浅的胎盘血肿。2.免疫荧光染色结果表明,在各日龄牛胎盘的尿囊绒毛膜、胎儿滋养层及母体子宫内膜均检测到IFNAR1的表达,阳性反应主要出现于尿囊绒毛膜上皮、子宫内膜上皮、子叶、母体子宫固有层内子宫腺及腺间结缔组织,其中IFNAR1在尿囊绒毛膜上皮、子宫内膜上皮及子宫腺呈强表达。免疫荧光相对表达量分析表明,IFNAR1在牛胎盘组织各部位的表达均随妊娠日龄增大而增强。3.qRT-PCR结果表明,在妊娠早期的尿囊绒毛膜和子叶组织,IFNAR1和IFNAR2 mRNA的表达随孕龄增大而不断增加,且IFNAR1 mRNA的表达水平整体上要高于IFNAR2的表达。结论:在奶牛妊娠早期,胎儿胎盘通过不断分枝、变长的胎儿尿囊绒毛膜绒毛增大与母体胎盘的子宫内膜上皮的嵌合而不断加大母胎之间的联系;妊娠早期牛胎盘组织中IFNAR1蛋白及IFNAR1和IFNAR2 mRANA呈规律性表达,提示两种受体在牛的早期妊娠维持和胎儿发育中发挥重要作用。研究结果不仅丰富了反刍动物胎盘组织形态学理论,也为胎盘滋养层细胞的相关基础和应用研究提供了基础形态资料,并为阐明IFNAR1和IFNAR在牛胎盘发育过程中的作用机制提供理论依据。
[Abstract]:Interferon - tau (IFNT) is a type I interferon, which is an anti luteinolytic factor.IFNT secreted by the gestational trophoblastic layer by combining its receptor IFNAR1 and IFNAR2 transmission signal or stimulating some factors to establish a maternal pregnancy recognition signal. At present, the IFNT receptor (IFNAR1 and IFNAR2) is present in the placental tissue of dairy cows. The study of expression and its role is not perfect. In this study, the microstructure of bovine placenta in early pregnancy was observed by paraffin section, HE and PAS staining techniques to determine the structural characteristics and development characteristics of the plate and caruncle of the fetus and to form the tissue structure of the bovine placenta in the early pregnancy; further through immunofluorescence staining The location and expression of IFNAR1, IFNAR protein and mRNA in bovine placenta in early pregnancy were studied by color and qRT-PCR techniques. The functional significance of IFNAR1 and IFNAR in the development of bovine placenta in early pregnancy was analyzed. The results were as follows: 1. the bovine placenta in the early pregnancy was cotyledon / upper chorion placenta and the caruncle of the uterus was a semicircular dome. With the increase of gestational age, the chorionic villus of the fetal allantoin is continuously branched, longer, and increased with the endometrium epithelium of the mother body, thus increasing the relationship with the endometrium epithelium in the maternal placenta area. After 27D, the fetal chorionic epithelium consists of mononuclear cells and PAS dyed three types of mononuclear cells with different types of trophoblast. The migration of nuclear giant cell into the syncytial body formed by the fusion of caruncle lacuna and endometrium epithelial cells; pregnancy 50D, the results of.2. immunofluorescence staining of shallow placental hematoma showed that the expression of IFNAR1 was detected in the urinal chorionic villus, fetal trophoblastic and maternal endometrium at every day of age, and the positive reaction was main. In the urinal chorionic epithelium, the endometrium, the cotyledon, the endometrium and the connective tissue in the matrix of the mother's uterus, IFNAR1 is in the urinal chorionic epithelium, the endometrium epithelium and the uterus are strongly expressed. The expression of the immunofluorescence relative expression indicates that the expression of IFNAR1 in the various parts of the bovine placenta is increased with the age of pregnancy. The results of enhanced.3.qRT-PCR showed that the expression of IFNAR1 and IFNAR2 mRNA in the chorionic chorionic and cotyledon tissues of the early pregnancy increased with the increase of gestational age, and the expression level of IFNAR1 mRNA was higher than the expression of IFNAR2 on the whole. Conclusion: in the early pregnancy of the dairy cows, the fetal placenta is continuously branched and the fetal chorionic cashmere is longer. The relationship between Mao Zengda and the endometrium epithelium of the maternal placenta increases the relationship between the mother fetus and the mother fetus; the IFNAR1 protein and IFNAR1 and IFNAR2 mRANA in the bovine placenta tissue are regularly expressed in the early pregnancy, suggesting that the two receptors play an important role in the maintenance of early pregnancy and the development of the fetus. The results not only enrich the ruminant animals, but also enrich the ruminant. The theory of placental histomorphology also provides basic morphological data for the related basis and application of placental trophoblast cells, and provides a theoretical basis for elucidating the mechanism of IFNAR1 and IFNAR in the development of bovine placenta.

【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823

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