丹翘灌注液抗炎镇痛作用及抗炎机理研究

发布时间：2018-04-23 11:49

  本文选题：丹翘灌注液 + 炎症模型　； 参考：《甘肃农业大学》2015年硕士论文

【摘要】：子宫内膜炎是奶牛的常发病之一,多发于产后,是子宫内膜感染引起的炎症,常导致奶牛不孕。奶牛患该病致使产犊间期延长,受孕率、妊娠率降低,产奶量下降,治疗费用和淘汰率增加,这给奶牛养殖业造成了巨大的经济损失。目前多采用抗生素、激素等治疗奶牛子宫内膜炎,但随着抗菌药物、激素等的长期大剂量应用常造成奶中药物残留,食用后危及人类健康。中药有提高动物机体免疫机能、抵抗疾病的能力,因其毒副作用小、残留少、特异性强成为人们积极探索的方向。丹翘灌注液是根据中兽医学理论和子宫内膜炎的病理特点研制而成的用于防治奶牛子宫内膜炎的中药制剂,但丹翘灌注液对奶牛子宫内膜炎是否具有抗炎作用,其抗炎作用机制如何?本研究从以下3个方面对丹翘灌注液的体内、体外抗炎、镇痛作用及其机制进行了研究,以期为临床试验用药及进一步研发抗炎中药制剂提供理论基础和依据。1、丹翘灌注液的体内抗炎作用研究。采用二甲苯致耳廓肿胀实验、醋酸致毛细血管通透性增加实验、棉球致肉芽肿增生实验评价其体内抗炎作用。结果显示,40g/kg丹翘灌注液对二甲苯致耳廓肿胀有极显著抑制作用、40g/kg、20g/kg丹翘灌注液对醋酸致毛细血管通透性增加、棉球致肉芽肿增生有显著抑制作用,表明40g/kg以上的丹翘灌注液对急、慢性炎症都有一定的抑制作用。2、丹翘灌注液的体内镇痛作用研究。采用醋酸诱导扭体反应和福尔马林致痛实验评价其镇痛作用。结果显示,40g/kg、20 g/kg丹翘灌注液对醋酸诱导的扭体反应、福尔马林致痛第Ⅱ相疼痛有显著的抑制作用。表明丹翘灌注液的镇痛活性主要参与外周炎症镇痛。3、丹翘灌注液的体外抗炎作用及抗炎机制研究。利用LPS刺激小鼠单核/巨噬细胞RAW 264.7建立体外炎症模型,研究丹翘灌注液对炎症模型细胞的炎性介质和炎性细胞因子(NO、PGE2、TNF-α和IL-6)的影响,以及对NF-κB信号通路的影响。结果显示丹翘灌注液(600μg/m L、300μg/m L、100μg/m L)作用于炎性细胞后能显著抑制LPS诱导的NO的分泌,TNF-α、IL-6、i NOS和COX-2基因的表达及胞核内NF-κB P65蛋白的表达,且具有一定的剂量效应,只有600μg/m L的丹翘灌注液对PGE2、TNF-α和IL-6的分泌显著抑制。结果表明丹翘灌注液对LPS诱导的炎症反应具有保护作用,其抗炎作用可能与调节NF-κB信号通路有关,主要是抑制炎性过程中关键酶和炎症因子的基因表达及炎症介质的产生。然而,丹翘灌注液作用炎性细胞后,炎性细胞因子IL-6及炎症限速酶i NOS和COX-2基因的表达与正常对照组仍存在显著差异,说明丹翘灌注液有抗炎作用,但不能完全阻断细胞炎症反应。
[Abstract]:Endometritis is one of the most common diseases in dairy cows. It is a kind of inflammation caused by endometrial infection and often leads to infertility. The disease causes prolonged calving interval, lower pregnancy rate, lower milk yield, higher treatment cost and higher elimination rate, which has caused enormous economic losses to the dairy cattle breeding industry. At present, antibiotics and hormones are used to treat endometritis in dairy cows. However, with the long-term use of antibiotics and hormones, the drug residues in milk are often caused, and human health is endangered after consumption. Traditional Chinese medicine has the ability to improve the immune function of animal body and resist disease. Because of its small toxicity, little residue and strong specificity, it has become an active exploration direction. Danqiao Perfusion liquid is a traditional Chinese medicine preparation for the prevention and treatment of endometritis in dairy cows, according to the theory of traditional Chinese veterinarian science and the pathological characteristics of endometritis. However, does Danqiao Perfusion liquid have anti-inflammatory effect on endometritis of dairy cows? What is the mechanism of its anti-inflammatory effect? In this study, the anti-inflammatory and analgesic effects and mechanism of Danqiao perfusion solution in vivo and in vitro were studied in the following three aspects. In order to provide theoretical basis and basis for clinical trial and further research and development of anti-inflammatory traditional Chinese medicine preparation. 1. In vivo anti-inflammatory effect of Danqiao perfusion solution. Auricular swelling induced by xylene, capillary permeability increase induced by acetic acid, granulomatous hyperplasia induced by cotton ball were used to evaluate its anti-inflammatory effect in vivo. The results showed that 40 g / kg Danqiao perfusion solution could significantly inhibit the auricle swelling induced by xylene. 40 g / kg danqiao perfusion solution could significantly inhibit the capillary permeability induced by acetic acid and the granuloma proliferation induced by cotton ball. It indicated that the perfusion solution above 40g/kg had an acute effect on auricle swelling. Chronic inflammation has a certain inhibitory effect. 2. Study on the analgesic effect of Danqiao Perfusion liquid in vivo. The analgesic effect was evaluated by acetic acid-induced writhing reaction and formalin induced pain test. The results showed that 40 g 路kg ~ (-1) 路kg ~ (-1) of 40 g 路kg ~ (-1) 路kg ~ (-1) 路min ~ (-1) g/kg could significantly inhibit acetic acid-induced writhing and formalin induced pain in phase 鈪，

本文编号：1791855