三七皂苷S-6的溶血性及体外免疫活性研究

发布时间：2018-04-23 12:45

本文选题：S- + 溶血性　；参考：《中国动物传染病学报》2017年04期

【摘要】：研究三七皂苷S-6的溶血性及体外免疫活性。常规方法提取PNS,大孔树脂柱层析分离纯化得S-6。溶血试验测定红细胞溶血率。脾淋巴细胞毒性试验确定S-6的安全剂量范围,体外脾淋巴细胞增殖反应和IL-2的诱生及含量测定检测S-6的体外免疫活性。S-6溶血性较小,2 mg/mL浓度以下无溶血性;S-6(0.1μg/mL、1μg/mL)显著促进了ConA诱导的小鼠脾淋巴细胞增殖反应,且与PNS和APS组相比,差异不显著;S-6(1μg/mL)极显著地促进了LPS诱导的小鼠脾淋巴细胞增殖反应,且与PNS相比,S-6(1μg/mL)差异显著,其余各个剂量与PNS和APS组相比,差异不显著;S-6(12.5μg/mL、25μg/mL、50μg/mL)显著或极显著地提高了IL-2的含量,且与PNS和APS组相比,差异不显著。S-6安全性好,体外具有较好的细胞免疫活性和诱生细胞因子的能力。
[Abstract]:To study the hemolysis and in vitro immune activity of Panax notoginsenoside S-6. PNSs were extracted by conventional method and purified by macroporous resin column chromatography. The hemolysis rate of red blood cells was measured by hemolysis test. The safety dose range of S-6 was determined by splenic lymphocyte toxicity test. Proliferation of splenic lymphocytes in vitro and induction and determination of IL-2 in vitro the in vitro immunological activity of S-6. S-6 hemolytic activity. S-6 hemolytic activity was less than 2 mg/mL. No hemolytic S-6N 0.1 渭 g / mL 1 渭 g 路mL ~ (-1) significantly promoted the proliferation of spleen lymphocytes induced by ConA in mice. Compared with the PNS and APS groups, the difference was not significant (P < 0.05). The proliferation of splenic lymphocytes induced by LPS was significantly promoted by S-6N (1 渭 g / mL), and the difference was significant compared with that of PNS (P < 0.05). The other doses were compared with those of PNS and APS groups. There was no significant difference in S-6, 12.5 渭 g / mL, 25 渭 g 路mL ~ (-1) or 50 渭 g / mL). Compared with PNS and APS, the difference was not significant. S-6 was safe, and had better cellular immune activity and the ability to induce cytokines in vitro.
【作者单位】：江苏农牧科技职业学院江苏省兽用生物制药高技术研究重点实验室;扬州大学兽医学院;
【基金】：江苏省兽用生物制药高技术研究中心重点实验室开放基金资助项目江苏省产学研合作项目“新型中兽药免疫增强剂复方桑杜口服液的研制”项目(BY2015066-01) 江苏高校“青蓝工程”资助 “泰州市311人才工程”项目学院“凤凰人才工程”项目
【分类号】：S853.7

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