整合REV LTR的自然重组马立克氏病毒GX0101的全基因组序列分析

发布时间：2018-04-23 19:38

本文选题：REV + LTR　；参考：《中国兽药杂志》2017年10期

【摘要】：GX0101是一株整合禽网状内皮组织增生症病毒(REV)长末端重复序列(LTR)的重组马立克氏病病毒(MDV)。基于MDV GX0101的细菌人工染色体感染性克隆利用高通量测序技术完成了对其全基因组的序列分析。GX0101全基因组长178101 bp,其基因组的TRL、UL、IRL、IRS、US和TRS区分别长12758 bp、113572 bp、12741 bp、12700 bp、11695 bp、13134 bp。与Md5不同,GX0101含有一个sorf2基因。同时,GX0101全基因组含有一个REV LTR重组片段,位于其基因组US区的sorf1中,sorf2基因前267 bp碱基处。通过与已发表的近10株MDV的比较,发现GX0101与英国分离株C12/130的两个不同毒力的感染性克隆p C12/130-10和p C12/130-15同源性最高。GX0101的全基因组序列的比较分析,有助于进一步阐明MDV致病性、传播性相关的基因,也有助于揭示不同地域间MDV的遗传变异和演化关系。
[Abstract]:GX0101 is a recombinant Marek's disease virus that integrates the long terminal repeat sequence of avian reticuloendotheliosis virus (Rev). Bacterial artificial chromosome infection clone based on MDV GX0101 was sequenced by high-throughput sequencing technique. The whole genome length of GX0101 was 178101 BP, and the length of IRS-US and TRS region was 12758 BP, 113572 BP, 12741 BP, 12700 BP, 11695 BP, 13134 BP, respectively. Different from Md5, GX0101 contains a sorf2 gene. At the same time, the whole genome of GX0101 contained a recombinant REV LTR fragment located at the 267bp base of sorf2 gene in the sorf1 of its US region. By comparing with nearly 10 MDV strains published, we found that the comparative analysis of the whole genome sequence of two infectious clones with different virulence, pC12 / 130-10 and pC12 / 130-15, with the highest homology of pC12 / 130-15, between GX0101 and British isolate C12 / 130, was helpful to further elucidate the pathogenicity of MDV. The transmissible genes also help to reveal the genetic variation and evolution of MDV in different regions.
【作者单位】：山东农业大学动物科技学院;山东省农科院;
【基金】：国家自然科学基金青年科学基金项目(31402235)
【分类号】：S852.65

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