鸭SCD1基因启动子多态性对血清生化指标的遗传效应

发布时间：2018-04-24 03:04

本文选题：鸭 + SCD基因　；参考：《南方农业学报》2017年10期

【摘要】：【目的】分析鸭SCD1基因启动子多态性与血清生化指标的遗传效应,揭示鸭SCD1基因启动子的生理调控机制,为今后开展畜禽SCD1基因研究及遗传标记选育提供参考依据。【方法】构建基因组DNA池,利用PCR-RFLP结合DNA直接测序技术检测鸭SCD1基因启动子区遗传变异情况,采用Meg Align寻找SNP位点,通过在线启动子分析软件预测SNP位点对鸭SCD1基因启动子g.952260~g.954527区域转录因子的影响,并以SPSS 19.0分析启动子酶切位点变异对父母代樱桃谷鸭血清生化指标的遗传效应。【结果】从樱桃谷鸭SCD1基因启动子g.952260~g.954527区域共检测到11个SNPs位点。其中,g.952323CA和g.952661AG位点突变分别致使原有转录因子D1和Sp1消失;g.952702AG位点突变则产生新的转录因子TEC1;g.952591TC、g.952868AT、g.952869TG、g.952971GC和g.953301TC突变区域无转录因子结合位点,也未产生新的转录因子结合位点;g.952873TG和g.954401TC位点突变未引起转录因子改变;g.954239CT位点突变导致原有转录因子Sp1、Sp1、Tra-1和AP-2α改变为Sp1、Tra-1、ETF和Sp1。通过PCR-Bgl II-RFLP和直接测序比对分析,发现g.952868AT和g.952869TG双碱基突变造成原始序列AGT_(952868)G_(952869)CT突变成AGA_(952868)T_(952869)CT,而产生新的Bgl II酶切位点,共产生3种基因型:CC(2268 bp)、CD(2268+1659+609 bp)和DD(1659+609 bp),2个等位基因(C和D)。CC基因型和C等位基因分别为优势基因型和优势等位基因,其频率分别为0.710和0.805;有效等位基因数(Ne)和多态信息含量(PIC)分别为1.458和0.265,属中度多态位点;卡方(χ~2)检验结果表明,Bgl II酶切位点突变所产生的基因型分布严重偏离哈代—温伯格平衡(χ~2χ_(0.01)~2,P0.01)。Bgl II酶切位点变异与父母代樱桃谷鸭血清生化指标的关联性分析结果表明,总体上以DD基因型樱桃谷鸭的血清生化指标略优于其他两种基因型。【结论】樱桃谷鸭SCD1基因启动子g.952260~g.954527区域共筛查到11个SNPs位点,其中g.952868AT和g.952869TG位点变异产生新的Bgl II酶切位点,且该酶切位点变异可能对鸭血清生化指标有调控效应。
[Abstract]:[objective] to analyze the genetic effect of duck SCD1 gene promoter polymorphism and serum biochemical index, and to reveal the physiological regulation mechanism of duck SCD1 gene promoter. [methods] Genomic DNA pool was constructed, PCR-RFLP combined with DNA direct sequencing technique was used to detect the genetic variation of duck SCD1 gene promoter region, and Meg Align was used to search for SNP locus. The effect of SNP locus on the transcription factors of g.952260~g.954527 region of duck SCD1 gene promoter was predicted by on-line promoter analysis software. SPSS 19.0 was used to analyze the genetic effect of promoter restriction site variation on the biochemical indexes of the blood of Cherry Valley duck. [results] A total of 11 SNPs loci were detected from the g.952260~g.954527 region of the SCD1 promoter of Cherry Valley duck. Among them, the mutation at the locus of G. 952323 CA and g.952661AG caused the mutation of the original transcription factor D1 and Sp1 to disappear. The mutation at the locus of g. 952702 AG produced a new transcription factor TEC1. 952591 TCU. 952868 ATG. 952869 TGN. 952971 GC and g.953301TC mutation region had no transcription factor binding sites. No new transcriptional factor binding sites, such as G. 952873 TG and g.954401TC, were found. No changes in transcription factors were found. The mutation of the original transcription factors Sp1, SP1, Tra-Tra-1 and AP-2 伪 resulted in the changes of the original transcription factors, Sp1P1-Tra-1ETFs and Sp1. By PCR-Bgl II-RFLP and direct sequencing alignment analysis, it was found that g.952868AT and g.952869TG double base mutations caused the original sequence AGT_(952868)G_(952869)CT to mutate into AGAS / s 952868 / CTT, resulting in the production of new Bgl II restriction sites. There were three genotypes: CC-2268bpC2268 1659 609bp) and DD(1659 609bp1. The two alleles were dominant genotype and dominant allele C, D).CC genotype and C allele, respectively, and the two alleles were dominant genotypes and dominant alleles, respectively. Its frequencies were 0.710 and 0.805, and the effective alleles and polymorphic information content (Pi) were 1.458 and 0.265, respectively, which were moderately polymorphic. The results of chi-square test (蠂 ~ 2) showed that the genotype distribution of BGL II restriction site mutation deviated seriously from the Hardy-Weinberg equilibrium (蠂 ~ 2 蠂 ~ 2 蠂 ~ (2) 蠂 ~ (2)) (蠂 ~ (2) 蠂 ~ (2) 蠂 ~ (2)) (蠂 ~ (2) 蠂 ~ (2)) (蠂 ~ (2) 蠂 ~ (2)). In general, the serum biochemical indexes of Cherry Valley duck with DD genotype were slightly superior to those of the other two genotypes. [conclusion] there were 11 SNPs loci screened in the g.952260~g.954527 region of the SCD1 promoter of Cherry Valley duck, in which g.952868AT and g.952869TG loci mutated to produce new Bgl II restriction sites. Moreover, the variation of the restriction site may have a regulatory effect on the biochemical indexes of duck serum.
【作者单位】：贵州大学动物科学学院;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;六盘水市草地工作站;贵阳市花溪区农业局;
【基金】：教育部博士点基金项目(20115201120004) 贵州大学研究生创新基金项目(研农2015025)
【分类号】：S834

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