阿司匹林丁香酚酯的一代繁殖毒性研究

发布时间：2018-04-24 04:18

本文选题：阿司匹林丁香酚酯 + 生殖毒性　；参考：《中国兽药杂志》2017年02期

【摘要】：为了初步评价阿司匹林丁香酚酯(AEE)对亲代大鼠的生殖功能和子代早期发育的影响,将360只Wistar大鼠随机分成6组,分别为受试药物AEE高、中、低剂量组(剂量分别为498.0、124.5和31.1 mg·kg~(-1)·d~(-1)),阿司匹林组(剂量为68.4 mg·kg~(-1)·d~(-1)),丁香酚组(剂量为246.0 mg·kg~(-1)·d~(-1)),空白对照组(给予常规维持饲料)。各实验组均按设定剂量连续给药10周后,测定亲代大鼠主要的脏器系数、繁殖指数和生产指标以及子代(F1)仔鼠的出生指标和生长指标。结果显示,与空白对照组相比,高剂量的AEE影响胎鼠的生长(P0.05),并且高剂量AEE组大鼠的妊娠率、窝产仔数、出生活仔率和出生存活率都偏低,其中雄性仔鼠比例偏高;阿司匹林组妊娠大鼠和哺乳期胎鼠生长缓慢(P0.05);高剂量组AEE亲代大鼠的睾丸和附睾系数偏高(P0.05),阿司匹林和丁香酚组子代大鼠的附睾系数偏高(P0.05)。结果表明,在本试验条件下,AEE对大鼠繁殖毒性的最大无损害作用剂量为31.1 mg·kg~(-1)·d~(-1),对大鼠的最小有损害作用剂量为124.5 mg·kg~(-1)·d~(-1)。
[Abstract]:In order to evaluate the effect of aspirin eugenol ester (AEE) on the reproductive function and early development of offspring, 360 Wistar rats were randomly divided into 6 groups. The low dose group (498.0124.5 mg / kg) and the low dose group (31.1 mg / kg 路kg ~ (-1)) were treated with a dose of 498.0124.5 and 31.1 mg / kg / d respectively. The aspirin group (68.4 mg / kg) and the eugenol group (246.0 mg / kg / kg) were treated with dimethylpyridamole (DX) -1, respectively, and the blank control group was given routine maintenance diet. The main organ coefficient, reproduction index and production index, birth index and growth index of the offspring were measured after 10 weeks of continuous administration of the drug in each experimental group. The results showed that compared with the blank control group, high dose of AEE affected the growth of fetal rats (P 0.05), and the pregnancy rate, litter size, litter size, survival rate and birth survival rate of the rats in the high dose AEE group were lower than those in the control group, in which the proportion of male rats was higher than that of the control group. The testis and epididymis coefficients of AEE parental rats in aspirin group and eugenol group were higher than that of control group, and the epididymal coefficients of offspring rats in aspirin and eugenol groups were higher than that in control group (P 0.05). The results showed that the maximum noninvasive dose of AEE on reproductive toxicity was 31.1 mg 路kg ~ (-1) 路d ~ (-1) D ~ (-1), and the minimum dose was 124.5 mg 路kg ~ (-1) 路d ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1) 路L ~ (-1) ~ (-1) 路L ~ (-1) ~ (-1) ~ (-1).
【作者单位】：中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室;
【基金】：国家自然基金项目(31572573)
【分类号】：S859.82

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