马立克氏病鸡组织中PRNP基因表达量分析

发布时间：2018-04-25 01:34

本文选题：鸡 + 马立克病　；参考：《甘肃农业大学学报》2017年04期

【摘要】：【目的】探明PRNP基因表达量与鸡马立克氏病发病之间的关系.【方法】从甘肃省康乐县某养鸡场采集马立克(MD)病鸡组织样品,通过RT-PCR检测MDV致瘤相关基因Meq和pp38以及病理组织学观察将样品分为MD肿瘤病变鸡、MD无病变鸡与健康鸡,最后通过荧光定量PCR方法分析3组中PRNP基因mRNA水平的相对表达量的差异.【结果】MD无病变鸡中PRNP基因的表达规律与健康鸡基本一致,但与MD肿瘤病变鸡完全不同;除脑外,MD病鸡组织PRNP基因的表达量均高于健康鸡,其中肿瘤病变组织PRNP基因的表达量显著高于无病变组织(P0.05),而且PRNP基因在MD病鸡肿瘤病变组织与健康鸡组织的表达差异与肿瘤病变程度有关.【结论】PRNP基因的表达量与MDV感染存在相关性,PRNP基因在MD肿瘤组织中过表达,且PRNP基因的差异表达与肿瘤病变程度有关.
[Abstract]:[objective] to investigate the relationship between the expression of PRNP gene and the incidence of Marek's disease. [methods] the tissue samples of Marek's disease were collected from a chicken farm in Kangle County, Gansu Province. The Meq and pp38 genes associated with MDV tumorigenesis were detected by RT-PCR and the samples were divided into two groups by histopathological observation. Finally, the relative expression of PRNP gene mRNA in three groups was analyzed by fluorescence quantitative PCR. [results] the expression pattern of PRNP gene in MD-free chickens was basically the same as that in healthy chickens, but it was completely different from that of MD-tumor-diseased chickens. The expression of PRNP gene in the tissues of MDD chickens except brain was higher than that in healthy chickens. The expression of PRNP gene in tumor tissues was significantly higher than that in non-diseased tissues (P 0.05), and the difference of expression of PRNP gene in MD diseased chicken tissues and healthy chicken tissues was related to the degree of tumor lesion. [conclusion] the expression of PRNP gene is related to the degree of tumor lesion. MDV gene was overexpressed in MD tumor tissues. The differential expression of PRNP gene was related to the degree of tumor lesion.
【作者单位】：甘肃农业大学动物医学院;
【基金】：国家自然科学基金项目(31160510,31500067) 甘肃省自然科学研究基金计划项目(1107RJZA198)
【分类号】：S858.31

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