亚急性瘤胃酸中毒对奶山羊血浆异常代谢产物及生化指标的影响

发布时间：2018-04-25 05:18

本文选题：亚急性瘤胃酸中毒 + 生化指标　；参考：《动物营养学报》2017年03期

【摘要】：本试验通过递增饲粮非纤维性碳水化合物与中性洗涤纤维比(NFC/NDF)方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA),旨在探讨SARA诱导过程中奶山羊血浆组胺(HIS)、脂多糖(LPS)含量及生化指标的变化。选取4只体况良好、体重接近的泌乳期萨能奶山羊,依次饲喂NFC/NDF为1.40、1.79、2.31、3.23的4种饲粮,每种饲粮饲喂15 d(为1组),前12 d为适应期,后3 d为采样期。酶联免疫吸附测定(ELISA)法检测血浆中HIS、LPS的含量,生化分析仪测定血浆生化指标。结果表明:1)当饲粮NFC/NDF由1.40递增至3.23时,成功诱导奶山羊处于SARA状态。2)在诱导SARA发生过程中,随着饲粮NFC/NDF增加,血浆HIS和LPS含量增加,NFC/NDF为3.23时显著高于NFC/NDF为1.40时(P0.05)。3)随着饲粮NFC/NDF增加,血浆免疫球蛋白M(Ig M)、免疫球蛋白A(Ig A)、免疫球蛋白G(Ig G)含量无显著变化(P0.05);血浆肌酐(CREA)、D-乳酸(LD)含量及肌酸激酶(CK)、谷草转氨酶(AST)、二胺氧化酶(DAO)活性无显著变化(P0.05);血浆γ-谷氨酰转移酶(γ-GT)活性呈升高趋势,NFC/NDF为3.23时显著高于其他各组(P0.05);血浆尿素氮(UN)含量降低,NFC/NDF为1.40时显著高于其他各组(P0.05);血浆碱性磷酸酶(ALP)活性升高,NFC/NDF为3.23时极显著高于其他3组(P0.01);血浆游离脂肪酸(FFA)含量先升高后降低,且在NFC/NDF为2.31时极显著高于其他各组(P0.01);血浆β-羟丁酸(β-HB)含量降低,NFC/NDF为1.40时呈极显著高于其他各组(P0.01)。结果提示,随着饲粮NFC/NDF递增,血浆中HIS和LPS含量显著增加,引发了机体炎症反应,加重了瘤胃上皮的损伤和SARA病情,激发奶山羊免疫活化状态;诱导SARA发生过程中,血浆生化指标有不同程度的变化,提示SARA期间奶山羊处于应激状态,引发了肝功能损伤。
[Abstract]:This experiment was conducted to induce subacute rumen acidosis in dairy goats by increasing dietary non-fiber carbohydrate to neutral detergent fiber ratio (NFC / NDF). The aim of this study was to investigate the plasma histamine, lipopolysaccharide (LPS) and its contents in dairy goats during the induction of SARA. Changes in biochemical indicators. Four suckling Sanen goats with good body condition and similar body weight were selected and fed with four diets (NFC/NDF 1.401.79 ~ 2.31 ~ 3.23). Each diet was fed for 15 days (group 1, the first 12 days as the adaptation period, and the latter 3 days as the sampling period). The content of HIS-LPS in plasma was detected by Elisa and biochemical index was determined by biochemical analyzer. The results showed that when dietary NFC/NDF increased from 1.40 to 3.23, the dairy goats were in SARA state. 2) in the process of inducing SARA, with the increase of dietary NFC/NDF, Plasma HIS and LPS contents increased significantly when NFC / NDF = 3.23 was significantly higher than that at NFC/NDF 1.40 (P 0.05 路3) with increasing dietary NFC/NDF. There was no significant change in plasma immunoglobulin (M(Ig), immunoglobulin A (Ig), immunoglobulin G (G(Ig) content (P0.05), but no significant change in plasma creatinine creatinine (creatinine), creatine kinase (CK), glutamic oxalacetic transaminase (ASTX), diamine oxidase (DAO) activity (P0.05N). The activity of 纬 -GTase (纬 -GT) increased with the increase of NFC / NDF (3.23), significantly higher than that of other groups (P0.05N); the decrease of plasma urea nitrogen (NDF) content was significantly higher than that of other groups (P0.05N); the activity of plasma alkaline phosphatase (ALPDF) increased significantly when NFC / NDF was 3.23 (P < 0.05); the activity of plasma alkaline phosphatase (ALP) was significantly higher than that of other groups (P = 3.23). In the other three groups, the content of FFAs in plasma increased first and then decreased. The plasma 尾 -hydroxybutyric acid (尾 -HBs) content was significantly higher than that of other groups when the NFC/NDF was 2.31 and the plasma 尾 -hydroxybutyric acid (尾 -HBs) content was 1.40. The level of plasma 尾 -hydroxybutyric acid (尾 -HBs) was significantly higher than that of other groups (P 0.01). The results suggested that with the increase of dietary NFC/NDF, the contents of HIS and LPS in plasma increased significantly, which caused inflammatory reaction, aggravated the injury of rumen epithelium and SARA, stimulated the immune activation of dairy goats, and induced SARA to occur. The changes of plasma biochemical indexes indicated that dairy goats were in stress state during SARA, which caused liver function damage.
【作者单位】：内蒙古农业大学动物科学学院;内蒙古农牧业科学院动物营养与饲料研究所;
【基金】：国家自然科学基金(31472124) 内蒙古自然科学基金(2015MS0380) 现代农业(奶牛)产业技术体系建设专项资金(CARS-37)
【分类号】：S858.27

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