工厂兔、散养兔和野兔盲肠菌群多样性及菌群结构分析

发布时间：2018-04-25 06:42

本文选题：肉兔 + 野兔　；参考：《家畜生态学报》2017年03期

【摘要】：论文旨在分析工厂兔、散养兔和野兔的盲肠菌群多样性及菌群结构。收集工厂兔、散养兔和野兔各6只并采集盲肠内容物,提取盲肠内容物基因组DNA并对16SrDNA的V4-V5区进行HiSeq测序。结果表明:(1)工厂兔盲肠细菌种类数、盲肠菌群丰富度、盲肠菌群多样性指数均显著(P0.05)高于散养兔和野兔。(2)工厂兔、散养兔和野兔盲肠菌群结构相互之间差异显著(P0.05)。(3)工厂兔、散养兔和野兔盲肠菌群的主要菌门为厚壁菌门(工厂兔为71.54%,散养兔为65.37%,野兔为63.25%)、拟杆菌门(工厂兔为23.78%,散养兔为30.8%,野兔为21.21%),野兔的特殊优势菌门为螺旋体门(12.36%)。工厂兔、散养兔和野兔盲肠菌群的主要菌科均含瘤胃菌科(工厂兔为39.26%,散养兔为33.33%,野兔为29.16%)、毛螺菌科(工厂兔为23.04%,散养兔为28.47%,野兔为21.54%),散养兔的特殊优势菌科为理研菌科(19.75%),野兔的特殊优势菌科为螺旋体科(12.36%)。在属水平上,工厂兔、散养兔和野兔盲肠菌群丰度最高的分别为拟杆菌属(9.45%)、理研菌科-RC9-gut-group(17.68%)、Sphaerochaeta(12.36%)。综上,工厂兔、散养兔和野兔盲肠菌群结构差异显著(P0.05),但主要菌门均为厚壁菌门和拟杆菌门;工厂兔盲肠菌群多样性高于散养兔和野兔。
[Abstract]:The purpose of this paper is to analyze the diversity and structure of cecum flora of factory rabbits, scattered rabbits and hares. The cecum contents were collected from the factory rabbits, 6 hare and 6 hare, and the genomic DNA of the cecum contents were extracted and the V4-V5 region of 16SrDNA was sequenced by HiSeq. The results showed that the number of cecum bacteria, the abundance of cecum flora, the diversity index of cecum flora in factory rabbits were significantly higher than those in scattered rabbits and hare. The main phylum of caecum flora of hare and hare were phylum thunbergii (71.54 in factory rabbits, 65.37 in scattered rabbits, 63.255um in hare), Bacteroides (23.78in factory rabbits, 30.88in scattered rabbits, 21.21th in hare), and 12.3636A in the special dominant bacteria phylum of hare. Factory rabbits, The main bacteriaceae of the cecum flora of scattered rabbits and hares contain ruminal bacteria (39.26 in factory rabbits, 33.33 in scattered rabbits, 29.1616A in hare, 23.04in factory rabbits, 28.4747 in scattered rabbits, 21.54 in hare), and the special dominant bacteriaceae in scattered rabbits is Rhizaceae. The special dominant bacteria of the hare is the helicidae 12.36. At the generic level, the highest abundance of the cecum flora of factory rabbits, scattered rabbits and hare were 9.45m of Bacteroides, and 17.68 of RC9-gut-group group of RC9-gut-group, and 12.36B of Sphaerochaeta. In conclusion, there were significant differences in the structure of cecum flora between factory rabbits, scattered rabbits and hare, but the main phylum were phylum thunbergiformis and Bacteroides phylum, and the diversity of cecum flora of factory rabbits was higher than that of scattered rabbits and wild rabbits.
【作者单位】：西北农林科技大学动物科技学院;
【基金】：[基金项目]家兔肉用性状功能基因解析(K3310216062) 伊高乐兔工厂化繁育技术(K3360216012)
【分类号】：S829.1

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