新种猪源嗜血支原体gapA基因的克隆及分子进化分析

发布时间：2018-04-27 09:31

  本文选题：猪附红细胞体 + 小附红细胞体　； 参考：《中国预防兽医学报》2017年08期

【摘要】：本实验室前期的流行病学调查中发现浙江省存在新种猪源嗜血支原体(CMh)的流行。为进一步确认该新种CMh,本研究设计兼并引物获得CMh gapA基因部分片段,通过Genome walking的方法获得了全基因cmh-gapA,大小为1 008 bp。基因序列比对分析表明cmh-gapA与犬M.haemocanis的gapA基因亲缘关系最近,同源性为74.6%。核苷酸序列的系统发育分析表明CMh与犬、猫的嗜血支原体位于同一分支,与M.suis和M.parvum亲缘关系较远。本研究为CMh作为新种猪源嗜血支原体提供佐证,并为猪源嗜血支原体GADPH蛋白功能研究和血清学诊断方法的建立奠定基础。
[Abstract]:An epidemiological investigation in our laboratory revealed the prevalence of new breed Mycoplasma haemophilus (CMH) in Zhejiang Province. In order to further identify the new species of CMH, a partial fragment of CMh gapA gene was obtained by designing degenerate primers, and the whole gene cmh-gapA was obtained by Genome walking with the size of 1 008 BP. The gene sequence alignment analysis showed that cmh-gapA had the closest relationship with the gapA gene of canine M.haemocanis, and the homology was 74.6. Phylogenetic analysis of nucleotide sequence showed that CMh was located in the same branch with dogs and cats, and was closely related to M.suis and M.parvum. This study provided evidence for CMh as a new breed of Mycoplasma sibiricum, and laid a foundation for the study of the function of GADPH protein and the establishment of serological diagnostic method for Mycoplasma suis.
【作者单位】： 浙江大学动物科学学院;浙江省农业科学院畜牧兽医研究所;绍兴市上虞区畜牧兽医技术推广中心;
【基金】：浙江省重点科技创新团队(2010R50027-26) 浙江省公益性项目(2017C32018)
【分类号】：S852.62
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本文编号：1810181