低剂量奶牛性控冷冻精液生产新技术产品试制与示范应用

发布时间：2018-04-28 06:16

本文选题：混合奶牛性控冷冻精液 + 精子活力　；参考：《内蒙古大学》2015年硕士论文

【摘要】：流式细胞仪分离精子的人工授精是目前控制哺乳动物出生后代性别最为可靠的繁育生物技术。但受专利和生产成本的限制,以奶牛为主的性控冷冻精液的高成本制约其广泛推广应用,因此提高奶牛性控冷冻精液精液的生产效率是当前降低产品成本的有效途径。本研究通过研制一种混合异种动物精液的新型奶牛性控冷冻精液生产技术,达到提高奶牛性控冷冻精液精生产效率、降低其生产成本并且可以进行产业化推广应用的目的。本实验对比了3种剂型混合异种动物精液的奶牛混合性控冷冻精液冻后活力、存活时间、顶体完整率检测结果及成年母牛人工授精效果。首先以低剂量(100万/0.25ml)奶牛分离X精子为性控冷冻精液产品目的精子,在分离X精液中添加不同浓度山羊精液,制备3个剂型的混合奶牛性控冷冻精液X1型、X2型、X3型(X1型含山羊精子300万个/0.25ml,X2型含山羊精子600万个／0.25ml,X3型含山羊精子900万个/0.25ml)。然后对3个剂型冻精活力、存活时间、顶体完整率进行评定,来探讨混合奶牛冷冻精液中不同浓度山羊精液对奶牛性控冷冻精液品质以及人工授精的受胎率影响。实验结果表明：X1型奶牛性控冷冻精液存活时间显著高于X2型和X3型产品(8 vs 6 vs 5) P0.05; X1型奶牛性控冷冻精液活力较X2型和X3型有所提高(55% vs 52% vs 48%),但相互之间差异不显著P0.05；X1型奶牛性控冷冻精液与X2型、X3型冻精的顶体完整率差异不显著(54% vs52.7% vs 51.9%) P0.05。但是,X1型奶牛性控冷冻精液进行人工授精90天后的受胎率显著高于X2型和X3冻精产品(51.9%vs33.6%vs29.9%) P0.05。根据上述实验结果提出本研究结论：浓度为300万/0.25ml的山羊精液混合100万/0.25ml的分离奶牛X精液制备的混合奶牛性控冷冻精液新技术产品,使生产效率提高2倍相对生产成本降低50%,冻精质量指标及人工授精的受胎率结果均达到常规性控冷冻精液的水平,证明该混合奶牛性控冷冻精液可以产业化生产并且进行产业化推广应用。
[Abstract]:The artificial insemination of separated sperm by flow cytometry is the most reliable reproductive biological technology to control the sex of the offspring of mammals. However, limited by the cost of the patent and production, the high cost of the frozen semen mainly controlled by the cow restricts its extensive application. Therefore, the efficiency of improving the production efficiency of the semen of milk cow's frozen semen is to be improved. The effective way to reduce the cost of the product is to improve the production technology of a new type of dairy cow sex controlled frozen semen, which is mixed with the semen of different species of animal, to improve the efficiency of the sperm production, reduce the cost of production and promote the popularization and application of the 3 kinds of mixture. The vitality, survival time, acrosome integrity test results and artificial insemination effect of adult cows were detected by mixed control frozen semen of physical semen. First, X sperm was isolated from dairy cows with low dose (1 million /0.25ml) as the target sperm of sex controlled frozen semen, adding different concentration of goat semen in the separation of X semen to prepare 3 dosage forms of mixed milk. Bovine sex controlled cryopreservation semen X1, X2, X3 (X1 type goat spermatozoa 3 million /0.25ml, X2 type goat spermatozoon 6 million / 0.25ml, X3 type goat spermatozoon 9 million /0.25ml), and then evaluate the vitality, survival time and acrosome integrity of 3 dosage forms of frozen semen to explore the performance of goat semen of different concentration in the frozen semen of mixed cows. The effects of the quality of frozen semen and the pregnancy rate of artificial insemination were controlled. The experimental results showed that the survival time of sex controlled frozen semen of X1 dairy cows was significantly higher than that of X2 and X3 type products (8 vs 6 vs 5) P0.05, and the activity of sex controlled cryopreservation semen in X1 dairy cows was higher than that of X2 and X3 (55% vs 52% vs 48%), but there was no significant difference between each other. There was no significant difference in the complete rate of the acrosome between the cow sex controlled cryopreservation semen and the X2 type and the X3 type freeze-cream (54% vs52.7% vs 51.9%) P0.05., but the abortion rate of the X1 type dairy cattle controlled frozen semen for 90 days was significantly higher than that of the X2 type and X3 cryopreservation product (51.9%vs33.6%vs29.9%) P0.05. based on the results mentioned above: the concentration was 3 million /0.25ml goat semen mixed with 1 million /0.25ml, a new technology product of mixed dairy cow sex controlled frozen semen prepared from dairy cow X semen, the production efficiency increased by 2 times the relative production cost by 50%, the quality index of the frozen semen and the artificial insemination rate reached the level of the conventional controlled cryopreservation semen, which proved that the mixed cow sex control was controlled. Frozen semen can be industrialized and popularized.

【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823

【参考文献】