重组UBC13对RAW264.7炎症因子mRNA表达的影响

发布时间：2018-04-28 11:02

本文选题：UBC + 小鼠巨噬细胞　；参考：《动物医学进展》2017年06期

【摘要】：为了研究重组蛋白UBC13对RAW264.7小鼠巨噬细胞炎症因子mRNA表达量的影响,采用噻唑蓝(MTT)法检测UBC13对RAW264.7细胞活力的影响,qPCR法检测不同浓度UBC13(20、10、5μg/mL)在不同时间点(3、6、9、12、24h)对RAW264.7细胞产生的IL-6、IL-1β、TNF-α和COX-2 4种炎症因子mRNA表达量的影响。结果表明,在整个试验时间范围内IL-6、IL-1β、TNF-α和COX-2mRNA表达量呈现逐步降低的趋势;与细胞对照组组相比,在24h时IL-6、IL-1β、TNF-α和COX-2mRNA表达量显著降低(P0.05)。说明重组蛋白UBC13作用于RAW264.7细胞24h时能够明显抑制RAW264.7细胞中IL-6、IL-1β、TNF-α和COX-2的mRNA表达,并且具有药物剂量依赖性。
[Abstract]:In order to study the effect of recombinant protein UBC13 on the expression of macrophage inflammatory factor mRNA in RAW264.7 mice, The effect of UBC13 on the viability of RAW264.7 cells was determined by using thiazolyl blue UBC13 assay. The effects of different concentrations of UBC1320C20 / 105 渭 g / mL) at different time points on the expression of IL-6, IL-1 尾 -TNF- 伪 and COX-2 four inflammatory cytokines mRNA were detected at different time points. The results showed that the expression of IL-6, IL-1 尾, TNF- 伪 and COX-2mRNA decreased gradually in the whole experimental time range, and the expression of IL-6, IL-1 尾, TNF- 伪 and COX-2mRNA decreased significantly at 24 h compared with the control group (P 0.05). The results showed that the recombinant protein UBC13 could inhibit the mRNA expression of IL-6, IL-1 尾, TNF- 伪 and COX-2 in RAW264.7 cells in a dose-dependent manner.
【作者单位】：山西农业大学动物科技学院;
【基金】：山西省科技攻关项目(20100312016)
【分类号】：S852.3

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