绵羊囊胚和孵化囊胚的转录组测序分析

发布时间：2018-04-29 12:54

本文选题：绵羊 + 囊胚　；参考：《新疆农业大学》2015年硕士论文

【摘要】：本文基于RNA-Seq技术,对绵羊的囊胚和孵化囊胚转录组进行了测序和分析,获得了一系列的数据资料,并对数据资料进行加工整理,得到了主要的研究结果如下:(1)通过Illumina/Hiseq2000测序平台对囊胚和孵化囊胚的转录组进行高通量测序,并对数据进行过滤、重组、注释等,分别得到47664034和47821016条Clean reads,比对到参考基因组上的reads分别达到71.48%和72.90%。(2)囊胚和孵化囊胚转录组分别得到了19318和18288个转录本,其中覆盖度达到90%-100%的转录本分别为8135和7823个。囊胚预测到2879个新转录本,孵化囊胚预测到1277个新转录本。(3)差异表达基因共有7700个,囊胚-VS-孵化囊胚表达上调基因3129个,表达下调基因4571个。(4)囊胚和孵化囊胚发生可变剪切的类型中外显子跳跃、内含子保留、5’端剪切位点替换、3’端剪切位点替换的数量分别为29117和11332,6864和5698,14899和8190,16321和9431个。(5)囊胚和孵化囊胚筛选出的SNP位点数量为97620和67471个,且筛选出的SNP类型中转换类突变明显要多于颠换类突变。测序结果中对基因的结构优化结果为:囊胚期优化基因数为5641个,孵化囊胚优化基因数5904个。本研究进行的绵羊转录组测序分析涉及转录组的基础数据分析和高级分析,为绵羊胚胎转录组的进一步研究提供了丰富的基础数据资料,并且为绵羊胚胎发育过程中基因的调控提供了一定理论基础,同时差异表达基因的筛选和分析反映了基因的表达情况,可以为相关生长过程中分子调控机理的深入研究提供基础。新转录本预测、可变剪切和SNP位点的筛选拓宽了绵羊转录组的相关信息,为进一步研究绵羊胚胎发育尤其是囊胚和孵化囊胚在着床前的过程中的基因调控提供了数据参考。
[Abstract]:Based on RNA-Seq technology, the transcriptome of blastocysts and hatched blastocysts of sheep were sequenced and analyzed. A series of data were obtained and processed. The main results are as follows: (1) High-throughput sequencing of blastocysts and hatched blastocysts by Illumina/Hiseq2000 sequencing platform, filtering, recombination, annotation, etc. 47664034 and 47821016 Clean transcripts were obtained respectively, and the reads compared to the reference genome reached 71.48% and 72.90.1%, respectively. 19318 and 18288 transcripts were obtained from blastocyst and hatched blastocyst transcriptome, respectively, of which 8135 and 7823 transcripts with coverage of 90-100% were obtained, respectively. The differentially expressed genes of blastocyst and hatched blastocyst were estimated to be 2879 new transcripts and 1277 new transcripts respectively. There were 7700 differentially expressed genes in blastocyst and 3129 up-regulated genes in blastocyst -VS- hatching blastocyst. The expression of down-regulated gene 4571. T4) the exon jumps in the types of blastocysts and hatched blastocysts with variable shear. The number of SNP site substitutions at the 3'end of the intron reserved 5 'end splicing sites were 29117 and 11332n6864 and 5698899 and 81908116321 and 9431 respectively) the number of SNP loci screened from blastocysts and hatched blastocysts were 97620 and 67471, respectively. Moreover, the transmutation of SNP was more than that of transverse-class mutation. The results of sequencing showed that the number of optimal genes in blastocyst stage was 5 641 and that of hatching blastocyst was 5 904. The analysis of sheep transcriptome sequencing in this study involves basic data analysis and advanced analysis of transcriptome, which provides abundant basic data for further study of sheep embryo transcriptome. It provides a theoretical basis for the regulation of genes during the development of sheep embryos, and the screening and analysis of differentially expressed genes reflect the expression of genes. It can provide the basis for the further study of molecular regulation mechanism in the process of growth. New transcripts predict that the screening of variable shear and SNP sites broadens the information of sheep transcriptome and provides a data reference for further study on the gene regulation of blastocysts and hatched blastocysts in sheep before implantation.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826

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