伪狂犬病病毒和猪圆环病毒2型混合人工感染仔猪排毒的检测

发布时间：2018-04-29 16:36

本文选题：仔猪 + 伪狂犬病病毒　；参考：《中国兽医科学》2017年03期

【摘要】：为了解伪狂犬病病毒(PRV)和猪圆环病毒2型(PCV2)感染仔猪后不同时间点的病毒载量及排毒规律,将34头健康仔猪分为4组,第1~3组分别为人工接种PRV、PCV2和PRV+PCV2组,第4组为空白对照组。分别在接种后第3、7、14、21、35天采集试验仔猪的鼻拭子和肛门拭子,用Taq Man荧光定量PCR(q PCR)对其进行定量检测,并分析仔猪排毒的变化规律。结果,接种后第3天,3个试验组仔猪肛门拭子、鼻拭子中均分离到PRV或PCV2;其中PRV单独感染后的病毒载量在第14天达到峰值331.67 copies/L;PCV2单独感染后的病毒载量在第3天达到峰值10 742.01 copies/L;PRV+PCV2混合感染后鼻拭子和肛门拭子的PRV分别在第14天和第7天达峰值980.96 copies/L和2 230.056 copies/L;鼻拭子和肛门拭子PCV2在感染后第14天达到峰值69.413 copies/L和271.72 copies/L;混合感染仔猪于感染后第8~14天发生死亡。3个感染组之间无论是肛门拭子还是鼻拭子,PRV、PCV2病毒载量均差异显著(P0.05)。上述结果表明,PRV单独感染、PCV2单独感染和二者混合感染均对仔猪造成严重危害,除引起仔猪发病死亡外,这2种病毒均能通过呼吸道和消化道向外排毒。
[Abstract]:In order to understand the viral load and detoxification rule of piglets infected with pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2) at different time points, 34 healthy piglets were divided into 4 groups. The fourth group was a blank control group. The nasal swabs and anal swabs of the piglets were collected on the 35th day after inoculation, and the quantitative Taq Man quantitative PCR(q was used to detect them, and the regularity of detoxification in piglets was analyzed. Results: on the 3rd day after inoculation, anal swabs of piglets in three experimental groups were obtained. PRV or PCV2 were isolated from nasal swabs, in which the viral load of PRV alone infected reached a peak value of 331.67 copiesL% PCV2 on the 14th day, and reached a peak value of 10 742.01 copies-L PRV PCV2 after mixed infection on the 3rd day after infection of nasal swabs and anal swabs. PRV reached a peak value of 980.96 copies/L and 2 230.056 copies-L on day 14 and day 7, PCV2 of nasal swab and anal swab reached a peak value of 69.413 copies/L and 271.72 copi / L on day 14 after infection, and death occurred on day 814 after infection in mixed infected piglets. There were significant differences in the viral load of PRV and PCV2 between anal swab and nasal swab (P 0.05). The results showed that both PCV2 and PCV2 infection alone caused serious harm to piglets, except for the death and morbidity of piglets, both of them could be discharged from the respiratory tract and digestive tract.
【作者单位】：云南农业大学动物科学技术学院;云南省动物疫病预防控制中心;晋宁县动物检疫和疫病预防控制中心;昆明伟牧得生物科技有限公司;
【基金】：云南省科技惠民计划项目(2013CH001) 昆明市科技合作重点项目(20130201AH023027)
【分类号】：S858.28

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