黑色素细胞中过量表达lpa-miR-nov-66对TYRP2的影响

发布时间：2018-05-02 22:17

本文选题：mircoRNA + lpa-miR-nov-　；参考：《中国兽医杂志》2017年10期

【摘要】：本研究旨在探讨lpa-miR-nov-66对黑色素生成相关基因TYRP2的影响。采用细胞转染技术,在羊驼黑色素细胞中过表达lpa-miR-nov-66,运用反转录聚合酶链反应(RT-PCR)、实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)技术和免疫印迹法(western blotting)技术分别检测转染lpa-miR-nov-66后黑色素细胞内TYRP2在mRNA和蛋白水平的表达差异。RT-PCR结果显示:TYRP2的部分片段扩增,扩增片段长度为151 bp。qRT-PCR和免疫印迹(western blotting)结果显示:与对照组相比,实验组TYRP2在mRNA表达水平显著降低,降低0.44倍;蛋白表达水平也显著降低,降低0.78倍。结果表明,lpa-miR-nov-66可以影响TYRP2的表达,可能参与了动物毛色的形成过程。
[Abstract]:The purpose of this study was to investigate the effect of lpa-miR-nov-66 on melanogenesis related gene TYRP2. Using cell transfection technology, Lpa-miR-nov-66 was overexpressed in melanocytes of alpaca. Reverse transcriptase polymerase chain reaction (RT-PCR), real-time quantitative real-time polymerase chain reactions-qRT-PCRand Western blotting were used to detect the expression of TYRP2 in melanocytes after lpa-miR-nov-66 transfection. The difference between mRNA and protein expression. RT-PCR results showed that the partial fragment of TYRP2 was amplified. The results of amplified fragment length of 151 bp.qRT-PCR and Western blotting showed that compared with the control group, the expression level of TYRP2 in the experimental group was significantly lower than that in the control group (0.44 times), and the protein expression level was also significantly decreased (0.78 times) in the experimental group. The results showed that lpa-miR-nov-66 could affect the expression of TYRP2 and might be involved in the formation of animal hair color.
【作者单位】：山西农业大学动物科技学院;河北省清河县农业局;
【基金】：国家“863”计划(2013AA102506) 公益性行业(农业)科研专项(201303119) 山西省研究生教育创新项目(2016BY071)
【分类号】：S852.2

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