德氏乳杆菌对育肥猪血脂指标、胆固醇代谢和脂肪沉积相关酶活性及基因mRNA表达的影响

发布时间：2018-05-03 05:31

本文选题：德氏乳杆菌 + 育肥猪　；参考：《动物营养学报》2017年09期

【摘要】：本试验旨在研究饲粮中添加德氏乳杆菌制剂对育肥猪血脂指标、肝脏胆固醇代谢和脂肪沉积相关酶活性、组织胆固醇代谢和脂肪沉积相关基因mRNA表达和皮下脂肪组织形态的影响。试验选用120头平均体重为(65.34±3.64)kg的健康"杜×(长×大)"育肥猪,随机分为2组,每组6个重复(栏),每个重复10头(公母各占1/2)。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加0.10%德氏乳杆菌制剂的饲粮,预试期7d,试验期42d。结果显示:1)与对照组相比,试验组育肥猪的血清甘油三酯(TG)(P=0.08)和总胆固醇(TC)(P=0.06)含量均有降低趋势,血清总胆汁酸(TBA)含量显著降低(P0.05)。2)与对照组相比,试验组育肥猪的肝脏羟甲基戊二酰辅酶A还原酶(HMGR)活性有降低趋势(P=0.07),胆固醇7α-羟化酶活性(CYP7A1)显著增加(P0.05),脂肪甘油三酯脂酶(ATGL)活性极显著降低(P0.01)。3)与对照组相比,试验组育肥猪的回肠胆汁酸结合蛋白(IBABP)的mRNA相对表达量有降低趋势(P=0.07),皮下脂肪过氧化物酶体增殖物激活受体γ(PPARγ)的mRNA相对表达量显著增加(P0.05);皮下脂肪细胞直径有降低趋势(P=0.09)。结果提示,德氏乳杆菌可通过干扰育肥猪回肠对胆汁酸的吸收来调控肝脏胆固醇和脂肪代谢。
[Abstract]:The aim of this study was to study the effects of Lactobacillus de on lipid index, liver cholesterol metabolism and lipid-deposition related enzyme activity in fattening pigs. Effects of tissue cholesterol metabolism and adipose deposition related gene mRNA expression and subcutaneous adipose tissue morphology. One hundred and twenty healthy pigs with an average body weight of 65.34 卤3.64)kg were divided into 2 groups randomly. They were divided into 2 groups, 6 replicates in each group (10 in each group) (male and female accounted for 1 / 2). The control group was fed with basic diet and the experimental group was fed with 0.10% Lactobacillus dermannii preparation in the basal diet for 7 days and 42 days respectively. The results showed that compared with the control group, the serum levels of TGG (0.08) and TC (0.06) in the experimental group were lower than those in the control group, and the serum total bile acid (TBAA) content was significantly lower than that in the control group (P 0.05N. 2). Compared with the control group, the liver HMGRs of the fattening pigs in the test group showed a tendency to decrease, while the activity of cholesterol 7 伪 -hydroxylase (CYP7A1) increased significantly, and the activity of fatty triglyceridase (ATGL1) decreased significantly compared with the control group. The relative expression of mRNA in ileal bile acid-binding protein (IBABP) of fattened pigs in the experimental group showed a decreasing trend, while the relative expression of mRNA in the subcutaneous adipoperoxidase proliferator activated receptor (纬 PPAR- 纬) increased significantly, and the diameter of subcutaneous adipocytes decreased. The results suggested that Lactobacillus de could regulate liver cholesterol and fat metabolism by interfering with the absorption of bile acids in the ileum of fattening pigs.
【作者单位】：湖南农业大学动物科学技术学院;湖南畜禽安全生产协同创新中心;
【基金】：国家科技计划课题(2013BAD21B04) 国家自然科学基金项目(31372322) 湖南省战略性新兴产业项目(2014GK1034)
【分类号】：S828.5

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