绵羊多羔主效基因FecB高通量检测方法的建立及应用

发布时间：2018-05-04 10:13

本文选题：FecB基因 + 产羔数　；参考：《畜牧兽医学报》2017年01期

【摘要】：基于前期分型基础,为了更进一步提高分型的效率和准确性,本试验建立了Taqman探针和SNaPshot两种高通量FecB突变分型方法。结果显示,与传统的PCR-RFLP和PCR-SSCP分型方法相比,这两种高通量分型方法在节省检测成本的同时,大大缩短了检测周期,并在检测效率上也有了大幅度的提高,对利用FecB进行分子育种具有潜在的应用价值。自2003年至今,本实验室应用这两种方法对10个中国本地绵羊品种、3个培育品种以及一些杂交群体的23 264只绵羊的FecB频率进行了检测,结果显示,湖羊和小尾寒羊携带FecB基因的频率最高,小尾寒羊FecB基因的B等位基因频率为0.432~0.833。跟踪以小尾寒羊作为母本和杜泊羊级进杂交后代群体发现,随着杂交的进行FecB基因在培育的鲁西黑头肉羊中的B等位基因频率提升到了0.432,但在横交固定阶段有所下降。本研究所得结果可为今后在肉用绵羊品种多羔改良和多羔肉用新品种培育过程中通过引入FecB基因提高繁殖性能提供参考数据。
[Abstract]:Based on the early typing foundation, in order to further improve the efficiency and accuracy of the typing, the Taqman probe and SNaPshot two high throughput FecB mutation classification methods are established in this experiment. The results show that compared with the traditional PCR-RFLP and PCR-SSCP classification methods, these two high throughput square methods greatly shorten the detection cost and greatly shorten the inspection. The test period has also been greatly improved in the detection efficiency. It has a potential application value for molecular breeding by using FecB. Since 2003, the laboratory has used these two methods to detect the FecB frequency of 10 Chinese native sheep breeds, 3 cultivar and 23264 sheep in some hybrid populations. The frequency of the FecB gene was the highest in Hu sheep and Small Tail Han sheep. The B allele frequency of the FecB gene of Small Tail Han sheep was tracked by 0.432~0.833., and the frequency of B allele in the mutton sheep of Luxi blackhead was increased to 0.432 with the hybridization of the FecB gene. The results of this study can provide reference data for improving reproductive performance by introducing FecB gene in the process of breeding new variety of lamb and multi lamb varieties for meat sheep breeds.

【作者单位】：中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与种质创新重点实验室;
【基金】：国家自然科学基金项目(31472078;31402041;31572371;31501941;31501926) 国家肉羊产业技术体系专项(CARS-39) 中央级公益性科研院所基本科研业务费专项(2013ywf-zd-1;2015ywf-zd-2;2015ywf-zd-8) 中国农业科学院科技创新工程(ASTIP-IAS13) 内蒙古自治区科技重大专项内蒙古自治区战略性新兴产业发展专项资金计划宁夏农林科学院科技创新先导资金项目(DWJLC-2016001)
【分类号】：S826

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