益生菌对雏鸡局部黏膜免疫功能及MUC2mRNA表达的影响

发布时间：2018-05-04 13:25

本文选题：雏鸡 + 消化道和呼吸道　；参考：《东北农业大学》2015年硕士论文

【摘要】：以1日龄雏鸡为研究对象,应用荧光定量PCR等技术结合酯酶染色法、甲基绿-派洛宁染色法、间接ELSIA法、阿利新兰-高碘酸希夫染色法(AB-PAS)等方法,通过MUC2mRNA表达及MUC2蛋白和免疫球蛋白含量,T淋巴细胞、浆细胞和杯状细胞数量等相关指标的动态检测,较全面系统的研究了不同种类益生菌应用对雏鸡消化道和呼吸道局部黏膜免疫组织(哈德尔腺、十二指肠、盲肠扁桃体)及局部体液免疫功能的影响,为益生菌在畜禽养殖业中的应用以及饲料用益生菌产品的研发提供重要的实验依据。研究主要结果如下:(1)雏鸡饲喂益生菌后,其哈德尔腺、十二指肠、盲肠扁桃体T淋巴细胞数量均程度不同的高于对照雏鸡。其中,10日龄时,哈德尔腺T淋巴细胞数量明显高于对照雏鸡(P0.05);7~13日龄时,十二指肠T淋巴细胞数量明显高于对照雏鸡(P0.05);10~21日龄时,盲肠扁桃体T淋巴细胞数量明显高于对照雏鸡(P0.05);表明益生菌可提高雏鸡局部黏膜组织的细胞免疫功能。(2)雏鸡饲喂益生菌后,其哈德尔腺、十二指肠、盲肠扁桃体的浆细胞数量均程度不同的高于对照雏鸡。其中,10~21日龄时,哈德尔腺浆细胞数量显著(P0.05或P0.01)高于对照雏鸡;7~13日龄时,十二指肠、盲肠扁桃体浆细胞数量(P0.05或P0.01)明显高于对照雏鸡;表明益生菌可提高雏鸡局部黏膜组织的体液免疫功能。(3)雏鸡饲喂益生菌后,其泪液、气管液、胆汁、肠液免疫球蛋白含量均不同程度的高于对照雏鸡。其中,7~13日龄时,泪液、胆汁免疫球蛋白含量均明显(P0.05或P0.01)高于对照雏鸡;4~21日龄时,气管液免疫球蛋白含量明显(P0.05或P0.01)高于对照雏鸡;4~13日龄时,肠液免疫球蛋白含量明显(P0.05或P0.01)高于对照雏鸡;表明益生菌可提高雏鸡局部体液免疫功能。(4)雏鸡饲喂益生菌后,其十二指肠、盲肠扁桃体杯状细胞数量以及MUC2mRNA表达量均高于对照雏鸡,其泪液、气管液、胆汁、肠液MUC2蛋白含量也高于对照雏鸡。其中,7~13日龄时,十二指肠、盲肠扁桃体杯状细胞数量和泪液中MUC2蛋白含量均明显(P0.05或P0.01)高于对照雏鸡;7~10日龄时,十二指肠MUC2m RNA表达和肠液中MUC2含量均明显(P0.05或P0.01)高于对照雏鸡;10~13日龄时,盲肠扁桃体MUC2mRNA表达和胆汁中MUC2蛋白含量均显著(P0.05)高于对照雏鸡;表明益生菌可促进局部黏膜组织杯状细胞的增殖,诱导其分泌更多的MUC2蛋白,促使MUC2基因表达上调,增强局部黏膜非特异性免疫功能。(5)乳酸杆菌单独应用,对雏鸡局部黏膜免疫功能及MUC2m RNA表达的作用效果优于双歧杆菌与乳酸杆菌1:1混合的益生菌制剂。
[Abstract]:The 1-day-old chicks were studied by using fluorescence quantitative PCR technique combined with esterase staining, methyl green-paronine staining, indirect ELSIA staining, and Alisinolan-periodate Schiff staining. The expression of MUC2mRNA and the contents of MUC2 protein and immunoglobulin in T lymphocytes, plasma cells and goblet cells were determined dynamically. The effects of different probiotics on the immune function of local mucosal tissues (Harder gland, duodenum, cecal tonsil) and local humoral immunity in chickens were studied. It provides an important experimental basis for the application of probiotics in livestock and poultry breeding and the research and development of probiotic products for feed. The main results were as follows: (1) the number of T lymphocytes in the tonsils of Harder gland, duodenum and cecum of chicks fed with probiotics was higher than that of control chickens. At the age of 10 days, the number of T lymphocytes in the Harder gland was significantly higher than that in the control chicks at the age of 7 and 13 days, and the number of T lymphocytes in the duodenum was significantly higher than that in the control chicks at the age of 1021 days. The number of T lymphocytes in the cecum tonsil was significantly higher than that in the control chicks P0.050.The results showed that probiotics could improve the cellular immune function of the local mucosal tissues of the chicks, and the Harder glands and duodenum of the chickens fed probiotics were increased. The number of plasma cells in the cecum tonsil was higher than that in the control. At the age of 10 ~ 21 days, the number of Harder adenoplasmacytes was significantly higher than that of control chicks (P 0.05 or P 0.01), and the number of tonsil cells in duodenum and cecum was significantly higher than that in control chicks (P 0.05 or P 0.01). The results showed that probiotics could improve the humoral immune function of local mucosal tissues of chicks.) after feeding probiotics, the contents of immunoglobulin in tear, trachea, bile and intestine were higher in chicks than those in control ones. The content of immunoglobulin in tear and bile was significantly higher than that in control chicks (P 0.05 or P 0.01) at day 13 (P 0.05 or P 0.01), and the content of immunoglobulin in trachea fluid was significantly higher than that in control chicks (P 0.05 or P 0.01). The content of immunoglobulin in intestinal fluid was significantly higher than that in control chicks (P0.05 or P0.01), which indicated that probiotics could improve the local humoral immune function of chicks. The number of goblet cells and the expression of MUC2mRNA in the cecum tonsil were higher than those in the control chicks, and the contents of MUC2 protein in tear, trachea fluid, bile and intestinal fluid were also higher than those in the control chicks. The number of goblet cells in duodenum and caecum tonsils and the content of MUC2 protein in tear were significantly higher than those in control chicks at age 713 (P0.05 or P0.01), and the number of goblet cells in duodenum and cecum was significantly higher than that in control chicks at 10 days of age. The expression of MUC2m RNA in duodenum and the content of MUC2 in intestinal fluid were significantly higher than those in control group (P0.05 or P0.01). The expression of MUC2mRNA in cecum tonsil and MUC2 protein in bile were significantly higher than those in control group (P 0.05). These results suggest that probiotics can promote the proliferation of goblet cells in local mucosal tissues, induce the secretion of more MUC2 proteins, promote the up-regulation of MUC2 gene expression, and enhance the local mucosal nonspecific immune function. The effect on local mucosal immune function and MUC2m RNA expression of chicks was better than that of the probiotics mixture of Bifidobacterium and Lactobacillus 1:1.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5

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