山羊CCRL2基因的分子克隆和表达分析

发布时间：2018-05-05 13:03

本文选题：山羊 + CCRL基因　；参考：《中国兽医学报》2017年11期

【摘要】：本研究旨在克隆山羊趋化因子(C-C基序)受体2(chemokine(C C motif)receptor-like 2,CCRL2)基因序列,并研究其组织和时序表达谱。以简州大耳羊为试验材料,应用RT-PCR方法克隆CCRL2基因并进行生物信息学分析,qPCR方法构建该基因在山羊不同组织表达谱和不同月龄背最长肌、股二头肌和臂三头肌中的表达水平,并将基因表达与IMF含量进行相关分析。结果显示:克隆得到山羊CCRL2基因(GenBank登录号:KT165378)长度为1 143bp,编码区长度为1 047bp;qPCR分析表明,CCRL2mRNA在24月龄山羊心、肝、脾、肺、肾、脂肪、背最长肌、股二头肌和臂三头肌中均有不同程度的表达,其中脾表达量最高(P0.01);时序表达中,CCRL2基因在股二头肌和臂三头肌中的表达量均在8~10月龄极显著高于1~3和24月龄(P0.01),在背最长肌中则为24月龄极显著高于1~3和8~10月龄(P0.01);相关分析显示,股二头肌中CCRL2基因mRNA表达量与IMF含量呈极显著负相关(P0.01)。CCRL2基因可能参与山羊IMF沉积作用。
[Abstract]:The aim of this study was to clone the gene sequence of goat chemokine C-C motif) receptor 2(chemokine(C C motif)receptor-like 2and CCRL2, and to study its tissue and temporal expression profile. The CCRL2 gene was cloned by RT-PCR and analyzed by bioinformatics to construct the expression profile of the gene in different tissues of goat and the expression level of longissimus dorsi muscle, biceps femoris muscle and triceps arm muscle at different months of age. The correlation between gene expression and IMF content was analyzed. The results showed that the length of goat CCRL2 gene was 1143 BP and the length of coding region was 1 047 BP / qPCR. The results showed that CCRL2 mRNA was found in 24 months old goat heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle. Both biceps femoris and triceps brachii showed different degrees of expression. The expression of CCRL2 gene in biceps femoris and triceps brachii muscle was significantly higher than that in the 1st and 24th months of age, and in the longissimus dorsi muscle, the expression of CCRL2 gene was significantly higher in the longissimus dorsi muscle than that in the 1st and 10th months of age, and the correlation analysis showed that the expression of CCRL2 gene in the biceps femoris and triceps brachii muscle was significantly higher than that in the longissimus dorsi muscle. The mRNA expression of CCRL2 gene in biceps femoris was negatively correlated with the content of IMF. CCRL2 gene may be involved in the deposition of IMF in goat.
【作者单位】：西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：四川省“十二五”畜禽育种攻关项目(2011NZ0099-36) 四川省科技创新产业链示范工程重大项目(2014NZ0003) 四川省教育厅项目(15ZA0385)
【分类号】：S827

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