山豆根多糖对PRRSV感染RAW264.7细胞存活率及分泌炎性因子的影响

发布时间：2018-05-07 01:29

本文选题：猪繁殖与呼吸综合征病毒(PRRSV) + 山豆根多糖　；参考：《南方农业学报》2016年12期

【摘要】：【目的】探讨山豆根多糖(SSP)对猪繁殖与呼吸综合征病毒(PRRSV)体外感染RAW264.7细胞存活率及分泌炎性因子水平的影响,为研发出治疗猪繁殖与呼吸综合征(PRRS)的新型兽药提供参考依据。【方法】以50、100、200和400μg/mL SSP作用于PRRSV体外感染8 h的RAW264.7细胞,通过MTT法评价SSP对PRRSV感染RAW264.7细胞存活率,ELISA检测SSP对PRRSV体外感染RAW264.7细胞培养上清液中的炎性因子TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1。【结果】PRRSV感染RAW264.7细胞8 h极显著降低了细胞存活率(P0.01,下同),而200~400μg/mL SSP能极显著升高PRRSV感染RAW264.7细胞存活率。PRRSV感染RAW264.7细胞8 h可极显著升高细胞分泌TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1水平,而SSP能有效降低PRRSV感染RAW264.7细胞分泌上述炎性因子水平,其中以200~400μg/mL SSP的抑制效果最佳。【结论】SSP通过提高炎症细胞存活率及抑制其分泌炎性因子水平,从而有效干预PRRSV感染免疫细胞的炎性反应。
[Abstract]:[objective] to investigate the effect of SSP on the survival rate of RAW264.7 cells infected with porcine reproductive and respiratory syndrome virus (PRRSVV) in vitro and the level of inflammatory cytokines. In order to provide a reference for the development of new veterinary drugs for the treatment of porcine reproductive and respiratory syndrome (PRRS). [methods] 50100200 and 400 渭 g/mL SSP were used to treat RAW264.7 cells infected with PRRSV for 8 h in vitro. MTT assay was used to evaluate the survival rate of RAW264.7 cells infected with SSP. Elisa was used to detect the inflammatory cytokines TNF- 伪, IL-1 尾, IL-6, IL-8, IL-10 and MCP-1in the supernatant of RAW264.7 cells infected with PRRSV in vitro by SSP. [results] the survival rate of RAW264.7 cells infected with PRRSV for 8 h was significantly lower than that of P0.01. However, 200 渭 g/mL SSP could significantly increase the survival rate of RAW264.7 cells infected with PRRSV for 8 h. The levels of IL-10 and MCP-1 in RAW264.7 cells secreted by RAW264.7 cells were significantly higher than those in RAW264.7 cells infected with PRRSV for 8 h, and the levels of IL-10 and MCP-1 in the cells secreted TNF- 伪, IL-1 尾, IL-6, IL-8 and IL-8 were significantly increased. However, SSP can effectively reduce the level of inflammatory cytokines secreted by RAW264.7 cells infected with PRRSV, and the inhibitory effect of 200 渭 g/mL SSP is the best. [conclusion] SSP can increase the survival rate of inflammatory cells and inhibit the level of inflammatory cytokines secreted by them. In order to effectively interfere with the inflammatory response of PRRSV infected immune cells.
【作者单位】：广西大学动物科学技术学院;广西中医药大学广西中医药科学实验中心;中国农业科学院蜜蜂研究所/农业部授粉昆虫生物学重点开放实验室;广西畜牧研究所;广西柯新源原种猪有限责任公司;
【基金】：国家自然科学基金项目(31260619) 广西高校科学技术研究项目(YB2014172)
【分类号】：S858.28

【相似文献】