低剂量多巴胺对肉鸡肾脏钠钾氯转运体（NKCC2）、钠氯转运体（NCC）、水通道蛋白2 （AQP2）表达的影响

发布时间：2018-05-08 14:56

本文选题：肉鸡 + 肾脏　；参考：《华中农业大学》2017年硕士论文

【摘要】：多巴胺是机体内最主要的儿茶酚胺类递质之一,随着人们对多巴胺作用机理的深入研究,发现许多疾病如抽动秽语综合征(Tourette综合征),精神分裂症,帕金森病等都与多巴胺神经递质传导有密切联系。低剂量多巴胺具有利尿利钠的作用,在人医临床上应用极为广泛,但在肉鸡此方面的研究尚未见报道。肾脏具有维持机体酸碱平衡、水盐代谢以及内环境稳态等功能,其主要是通过钠钾氯共转运体(NKCC2)、钠氯共转运体(NCC)和水通道蛋白2(AQP2)等对水盐代谢的调控来实现的。研究表明,低剂量多巴胺具有利钠利尿作用,对肾小管钠水的重吸收具有较强的抑制作用。为研究低剂量多巴胺对肉鸡肾脏钠钾氯共转运体(NKCC2)、钠氯共转运体(NCC)和水通道蛋白2(AQP2)表达的影响,本试验按常规条件饲养AA肉鸡40羽,25日龄时随机分为对照组(C组=20)和试验组(D组=20),试验组肉鸡经静脉注射给予低剂量左旋多巴胺(5μg/kg/min),对照组肉鸡按相同方法给予等体积0.9%生理盐水。并采用组织形态学观察、血清生化检测、蛋白质免疫印迹试验和RT-q PCR等方法,检测肉鸡肾组织形态学和各种生化指标的变化,及钠钾氯共转运体(NKCC2)、钠氯共转运体(NCC)和水通道蛋白2(AQP2)蛋白水平和m RNA水平的变化。结果显示:与对照组肉鸡相比,试验组在给以低剂量左旋多巴胺后,尿素氮、肌酐(Cre)及动脉压(m PAP)水平均未发生明显改变(P0.05);低剂量左旋多巴胺持续给药后,肉鸡肾小管上皮细胞胞膜NKCC2、NCC、AQP2蛋白水平显著降低,细胞质NKCC2、NCC、AQP2蛋白显著升高(P0.05);然而NKCC2、NCC、AQP2 m RNA水平未发生明显变化(P0.05)。本试验从肾脏水钠代谢途径中的关键水钠通道蛋白的表达变化着手,探讨了低剂量多巴胺对肉鸡水钠通道蛋白调节的机制。实验结果表明,在不影响肾小球滤过率及血压前提下,低剂量多巴胺能够引起肉鸡机体持续的利钠利尿作用,其机制与NKCC2、NCC、AQP2蛋白的穿梭作用有关。
[Abstract]:Dopamine is one of the most important catecholamine transmitters in the body. With the further study of the mechanism of dopamine, many diseases such as Tourette syndrome and schizophrenia have been found. Parkinson's disease is closely related to dopamine neurotransmitter conduction. Low dose dopamine has diuretic natriuretic effect and has been widely used in human medicine, but it has not been reported in broiler. The kidney has the functions of maintaining the balance of acid and base, water and salt metabolism, and the homeostasis of the internal environment, which is mainly realized by the regulation of water and salt metabolism by the sodium, potassium and chlorine co-transporters NKCC2, sodium chloride co-transporter NCC2, and aquaporin 2aquaporp. The results showed that low dose dopamine had diuretic effect and inhibited the reabsorption of renal tubular sodium water. To investigate the effects of low dose dopamine on the expression of NKCC2N, NCC2 and aquaporin 2AQP2in the kidney of broilers. In this study, 40 AA broilers were randomly divided into control group (group C) and control group (group D) at 25 days of age under conventional conditions. The experimental group was given low dose of L-dopamine 5 渭 g / kg / min by intravenous injection, while the control group was given the same method. The volume of saline was 0.9%. Histomorphologic observation, serum biochemical test, Western blot test and RT-q PCR were used to detect the changes of renal histomorphology and biochemical indexes in broilers. The changes of protein levels and m RNA levels of sodium potassium chloride co-transporter NKCC2, sodium chloride co-transporter NCC2 and aquaporin-2aquaporin (AQP2). The results showed that compared with the control group, the levels of urea nitrogen, creatinine (creatinine) and arterial pressure (MPP) in the experimental group were not significantly changed after the low dose of L-dopamine was given, and the low dose of levodopamine was given continuously. The protein level of NKCC2 / NCC-AQP2 in renal tubular epithelial cells of broilers was significantly decreased, while the protein level of NKCC2 / NCC-AQP2 was significantly increased in the cytoplasm of NCC2, but the level of NKCC2 / NCCP AQP2 m was not changed significantly (P 0.05). In this study, we studied the mechanism of low dose dopamine on the regulation of sodium channel protein in broilers by analyzing the changes of the expression of sodium channel protein in renal water sodium metabolism pathway. The results showed that, without affecting glomerular filtration rate and blood pressure, low dose dopamine could induce continuous diuretic natriuretic effect in broilers, and its mechanism was related to the shuttle effect of NKCC2NCC-AQP2 protein.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S859.7

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