贵阳动物园动物隐孢子虫的调查与虫种鉴定

发布时间：2018-05-08 23:30

本文选题：隐孢子虫 + S　；参考：《中国兽医科学》2017年03期

【摘要】：为了解贵阳动物园圈养野生动物隐孢子虫的感染流行情况,以骆驼、黑熊、斑马、袋鼠、猕猴、长臂猿、大象等野生动物的新鲜粪便为样品(n=84),采用饱和蔗糖溶液漂浮法收集隐孢子虫的卵囊并提取D N A,以18S r R NA为目标基因靶点设计特异性引物,并采用巢式PCR扩增目的基因,后经10 g/L琼脂糖凝胶电泳,对检测结果为阳性的PCR产物进行序列分析并建立系统发育树。结果表明,共有13份隐孢子虫阳性样品,其中骆驼7份,感染率为70.00%,感染虫种为安氏隐孢子虫;斑马1份,感染率为10.00%,感染虫种为安氏隐孢子虫;长臂猿5份,感染率为23.81%,感染虫种为微小隐孢子虫、人隐孢子虫及小鼠隐孢子虫。本次调查发现该园野生动物隐孢子虫具有较高的总体感染率(15.50%)和鉴定出4种人兽共患隐孢子虫虫种,具有较重要的公共卫生意义。本研究首次报道了我国斑马感染隐孢子虫。
[Abstract]:In order to understand the prevalence of cryptosporidium in captive wild animals in Guiyang Zoo, camels, black bears, zebras, kangaroos, macaques, gibbons, Fresh feces from wild animals such as elephants were used as samples. The oocysts of Cryptosporidium were collected by saturated sucrose solution floating method. The specific primers were designed with 18s r R NA as the target gene target, and the target genes were amplified by nested PCR. After 10 g / L agarose gel electrophoresis, the positive PCR products were sequenced and phylogenetic tree was established. The results showed that there were 13 positive samples of Cryptosporidium, of which 7 were camels, the infection rate was 70.00.The infection rate was Cryptosporidium andersoni, one zebra, the infection rate was 10.00, the infection rate was Cryptosporidium Angiosporium, and the gibbon was 5. The infection rate was 23.81%. The infected species were Cryptosporidium minimus, Cryptosporidium human and Cryptosporidium mouse. This investigation found that the wild animal Cryptosporidium has a higher total infection rate of 15.50%) and identified four species of zoonotic Cryptosporidium species, which has important public health significance. This study reported for the first time that zebras were infected with Cryptosporidium in China.
【作者单位】：四川农业大学动物医学院动物疫病与人类健康四川省重点实验室;重庆市石柱县畜牧产业发展中心西沱镇畜牧兽医站;成都大熊猫繁育研究基地;四川省名山区农业局;
【基金】：成都大熊猫繁育研究基金会项目(CPF2014-14) 国家自然科学基金项目(31272620)
【分类号】：S852.723

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