一株青贮用高性能乳酸菌的筛选

发布时间：2018-05-09 05:45

  本文选题：青贮饲料添加剂 + 外源菌粉　； 参考：《安徽农业大学学报》2017年04期

【摘要】：为筛选优良青贮饲料添加剂,从德国南部山区苜蓿制备菌粉中分离得到5株乳酸菌。通过对菌株形态学、生理生化和16S r RNA相结合方法进行鉴定,对5株外源乳酸菌和常规优势菌株7的生长曲线测定和产酸性试验,得到3株高性能乳酸菌,并对这3株乳酸菌的生物学特性进一步研究。结果显示,R1、R5为植物乳杆菌,R2、R3和R4为屎肠球菌;菌株R 1、7和R 5生长迅速、产酸能力较好;T50℃时,菌株R1比R5、7耐温能力强,pH2.5时,菌株R1的适应能力比菌株7、R5强,菌株R1比菌株R5、7抑菌能力强,外源品种R1可作为制备乳酸菌青贮饲料添加剂新的优良菌株。
[Abstract]:In order to screen fine silage additives, 5 strains of lactic acid bacteria were isolated from alfalfa powder in southern Germany. By the combination of morphology, physiology and biochemistry and 16s r RNA, the growth curve and acid production of 5 exogenous lactic acid bacteria and routine dominant strain 7 were determined, and 3 high performance lactic acid bacteria were obtained. The biological characteristics of these three strains of lactic acid bacteria were further studied. The results showed that R1 and R5 were Lactobacillus plantarum, R2, R3 and R4 as Enterococcus faecium, and strains R1 and R5 grew rapidly and had better acid production at T50 鈩，

本文编号：1864828