美洲大蠊提取物对大鼠卵巢卵泡发育的影响

发布时间：2018-05-09 07:04

  本文选题：美洲大蠊提取物 + 卵巢发育　； 参考：《中国畜牧杂志》2017年04期

【摘要】：实验旨在研究美洲大蠊提取物是否对大鼠卵巢卵泡发育产生影响。将成年SD大鼠随机分为3组,对照组(9 m L/kg,生理盐水)5只,低剂量组(9 m L/kg,0.03 g/m L美洲大蠊提取物)6只、高剂量组(9 m L/kg,0.09 g/m L美洲大蠊提取物)6只。每隔1 d灌胃一次,30 d后采集卵巢。结果表明:与对照组相比,低剂量组大鼠的卵巢重和卵巢体重比以及高剂量组大鼠的体重和卵巢重均显著增加(P0.05);高、低剂量组大鼠卵巢原始卵泡的数目均显著减少(P0.05),成熟卵泡显著增多(P0.05);生长发育相关基因BMP4、GDF9的表达显著上调(P0.05);低剂量组IGF-Ⅰ基因的表达有升高的趋势(P=0.058),但高剂量组则显著上调(P0.05);3个组间凋亡相关基因Bcl-2、BAX、Fas、Fas L和Caspase-3的表达均无显著性差异;但高剂量组使增殖相关基因的表达有升高的趋势(P=0.06),使Ki67基因的表达显著升高PCNA(P0.05)。本实验条件下,美洲大蠊提取物能够加快大鼠卵泡的发育,促进卵巢卵泡的成熟。
[Abstract]:The aim of this study was to investigate whether the extract of Blattella Americana had an effect on ovarian follicle development in rats. Adult Sprague-Dawley rats were randomly divided into three groups: control group (9 mL / kg), normal saline (n = 5), low dose group (n = 6) with 9 mL / kg ~ (- 0.03) g / mL Blattella Americana extract (n = 6) and high dose group (n = 9) with 9 mL / kg ~ (0.09 g / mL) Blattella Americana extract (n = 6). Ovaries were collected after 30 days of gastric perfusion every 1 day. The results showed that: compared with the control group, the ovarian weight and ovarian weight ratio of the low dose group and the high dose group were significantly higher than that of the control group, and the ratio of ovarian weight to ovarian weight in the low dose group was significantly higher than that in the high dose group. In low dose group, the number of primordial follicles in ovary decreased significantly, the number of mature follicles increased significantly, the expression of growth and development related gene BMP4 and GDF9 increased significantly, and the expression of IGF- 鈪，

本文编号：1865053