甲减和甲亢对青年大鼠卵巢和子宫氧化应激的影响

发布时间：2018-05-09 09:41

本文选题：甲状腺激素 + 抗氧化状态　；参考：《南京农业大学》2015年硕士论文

【摘要】：甲状腺激素(thyroid hormones)和氧化应激(oxidative stress)在雌性动物生殖系统的正常生理功能中发挥着重要作用。一氧化氮(nitric oxide,NO)是一种由一氧化氮合酶(nitric oxide synthases,NOS)催化合成的小分子自由基,可参与甲状腺功能和氧化应激状态的调节,也被认为是一个评价氧化应激状态的标记物。本研究的目的是研究甲状腺激素对青年大鼠卵巢和子宫氧化应激的影响。30只刚断奶的未成熟SD大鼠被随机分成三个组：对照组(Control),甲状腺功能减退(甲减)组(Hypo-T),和甲状腺功能亢进(甲究)组(Hyper-T)。使用丙硫氧密啶(propylthiouracil,PTU)和甲状腺激素(thyronine,T4)来分别构建甲减和甲亢大鼠模型。研究结果显示:甲减组和甲亢组大鼠的体重和卵巢重量都会显著降低,甲减组的子宫重量显著降低。甲减组和甲亢组卵巢中的卵泡数量也显著减少。血液中三磺甲状腺原氨酸(triiodothyronine,T3),甲状腺素(thyroxine,T4)和雌二醇(estradiol,E2)的浓度在甲减组大鼠中显著降低,而在甲亢组大鼠中会显著升高。此外,甲减组和甲亢组大鼠血液中的孕酮(progesterone,P4)浓度都显著降低。免疫组织化学结果表明:甲状腺激素受体(TRα/β)和三种NOS(nNOS、iNOS和eNOS)在大鼠卵巢和子宫的不同类型细胞中定位和表达,表明这些受体对卵巢和子宫的生长发育和功能起着重要作用。卵巢和子宫中的NO含量,总NOS活性,以及诱导型NOS(iNOS)活性在甲减组大鼠中显著降低,而在甲亢组大鼠中会显著升高。卵巢中结构型NOS(cNOS)活性在甲减组大鼠中最低,而在甲亢组和对照组大鼠之间没有显著差异。甲减组大鼠子宫中cNOS的活性要比甲亢组大鼠显著降低,但甲减组和甲亢组大鼠都与对照组大鼠差异不显著。卵巢和子宫中的丙二醛(malondialdehyde,MDA)含量在甲减组和甲亢组大鼠中都升高。卵巢和子宫中的过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-PX)的活性在甲减组大鼠中降低,而在甲亢组大鼠中都升高。卵巢中总超氧化物歧化酶(totalsuperoxide dismutase,T-SOD)的活性在甲亢组大鼠中最高,而在甲减组和对照组大鼠之间没有显著差异。子宫中的T-SOD的活性在甲减组和甲亢组大鼠中都比对照组显著降低,但在甲减组和甲亢组大鼠之间没有显著差异。综上所述,通过使用甲减甲亢大鼠模型进行研究,实验结果提供了甲状腺功能紊乱对青年大鼠卵巢和子宫中氧化应激有重要影响的新证据。但是,在甲状腺激素引起的生殖疾病中,NOS信号和氧化应激的作用仍有待进一步研究。值得注意的是,甲状腺激素与雌性生殖系统的抗氧化状态有重要联系,这些结果也有助于阐明甲状腺功能紊乱对雌性动物生殖的影响。
[Abstract]:Thyroid hormone thyroid hormones (thyroid hormones) and oxidative stress stress (oxidative stress) play an important role in the normal physiological function of female reproductive system. Nitric oxide (no) is a small molecular free radical catalyzed by nitric oxide synthase (oxide syntheses), which is involved in the regulation of thyroid function and oxidative stress state, and is also considered to be a marker for evaluating oxidative stress state. The purpose of this study was to study the effects of thyroid hormone on oxidative stress in ovary and uterus of young rats. Thirty newly weaned immature SD rats were randomly divided into three groups: control group (control group), hypothyroidism (hypothyroidism) group, and hypothyroidism (hypothyroidism) group. Hyperthyroidism group (Hyper-T) group. Rat models of hypothyroidism and hyperthyroidism were established by using propylthiouraciline (PTU) and thyroid hormone thyronine (T4) respectively. The results showed that the weight and ovarian weight of hypothyroidism group and hyperthyroidism group decreased significantly, while the uterine weight of hypothyroidism group decreased significantly. The number of follicles in hypothyroidism and hyperthyroidism was also significantly reduced. The concentrations of triiodothyronine (T3), thyroxine (T4) and estradiolium (E2) in blood decreased significantly in hypothyroidism group, but increased significantly in hyperthyroidism group. In addition, the serum progesterone P4 concentration in hypothyroidism group and hyperthyroidism group was significantly decreased. Immunohistochemical results showed that thyroid hormone receptor tr 伪 / 尾 and three NOSnNOSiNOS and Enos) were localized and expressed in different types of cells of rat ovary and uterus, indicating that these receptors play an important role in the growth, development and function of ovary and uterus. No content, total NOS activity and inducible NOSI-NOSs activity in ovary and uterus decreased significantly in hypothyroidism group, but increased in hyperthyroidism group. The activity of structural NOSc NOSs in ovary was the lowest in hypothyroidism group, but there was no significant difference between hyperthyroidism group and control group. The activity of cNOS in hypothyroidism group was significantly lower than that in hyperthyroidism group, but there was no significant difference between hypothyroidism group and hyperthyroidism group and control group. The content of malondialdehyde (MDA) in ovary and uterus was increased in hypothyroidism group and hyperthyroidism group. The activities of catalase (CAT) and glutathione peroxidase (glutathione peroxidase) in ovary and uterus decreased in hypothyroidism group and increased in hyperthyroidism group. The activity of total superoxide dismutase (T-SOD) in ovary was the highest in hyperthyroidism group, but there was no significant difference between hypothyroidism group and control group. The activity of T-SOD in uterus was significantly lower in hypothyroidism group and hyperthyroidism group than in control group, but there was no significant difference between hypothyroidism group and hyperthyroidism group. In conclusion, by using hypothyroidism rat model, the results provide new evidence that thyroid dysfunction has an important effect on oxidative stress in ovary and uterus of young rats. However, the role of nitric oxide synthase (NOS) and oxidative stress in thyroid hormone-induced reproductive diseases remains to be further studied. It is important to note that thyroid hormones are closely related to the antioxidant status of the female reproductive system. These results also help to clarify the effects of thyroid dysfunction on the reproduction of female animals.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S856.5

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