黄芪多糖粉和银黄粉缓解羊运输应激的研究

发布时间：2018-05-10 06:26

本文选题：黄芪多糖粉 + 银黄粉　；参考：《河北农业大学》2015年硕士论文

【摘要】：运输应激是一种复杂的受多方面因素影响的应激,羊在发生运输应激后所表现的状态一般被称为运输应激综合征。运输应激的发生会同时影响被运输羊的神经-内分泌系统、免疫功能、血液生理指标、血液生化指标以及生产性能。本试验以经过长途运输的小尾寒羊为试验动物,以黄芪多糖粉和可溶性银黄粉为试验药物,采用饮水给药的方式进行了为期14d的缓解应激试验。本试验通过测定经过长途运输后小尾寒羊在不同时间的各项与运输应激相关的指标以及发病情况和生产性能的变化,分析确定了试验药物对小尾寒羊运输应激有良好的缓解作用,同时确定了试验药物的最适用量,为生产实践中缓解羊的运输应激同时减少经济损失提供了相应依据。结果表明:经过长途运输试验羊在试验开始阶段均处在强烈的运输应激状态下,其各项生理生化指标均未在正常范围内。随着试验的进行试验羊的应激状态开始得到缓解。在生产性能方面,运用试验药物的试验组与未用药的对照组相比小尾寒羊的采食量与体重增加量均有显著的差异,最适用药量试验组羊的料肉比为4.89,明显优于对照组的5.80,表明试验组羊的生产性能明显好于对照组。试验羊在试验期间的血液生化指标的变化显示,最适用药量试验组羊的各项相关生化指标的恢复速度明显好于对照组,其中以肌酸激酶、乳酸脱氢酶以及皮质醇的含量变化最为显著。试验羊运达后的三大临床指标显示,试验组小尾寒羊的体温、脉搏和呼吸频率的恢复速度均明显优于对照组,可以更快的恢复到正常水平。试验羊在试验期间的血液常规指标的测定结果显示,经过运输后各组试验羊的白细胞总数均高于正常值,而淋巴细胞百分比却明显低于正常值,随着试验的进行白细胞总数开始逐渐降低而淋巴细胞百分比开始回升,最适用药量试验组羊的白细胞恢复速度明显优于对照组。试验期间试验羊的呼吸道疾病发病率和消化道疾病发病率明显降低,其中对照组羊的呼吸道疾病发病率和消化道疾病发病率分别为3.26%和4.59%,最适用药量组试验羊的相应发病率分别为1.12%和2.55%。结论:经过长途运输处于应激状态的小尾寒羊,通过黄芪多糖粉和银黄可溶性粉的联用可以调节其机体的生理功能,改善临床指标以及生理生化指标,在提高生产性能的同时降低发病率,对缓解羊的运输应激有良好的效果。本试验中黄芪多糖粉和银黄粉的最佳用量为黄芪多糖粉1.0g/只/天+银黄可溶性粉1.0g/只/天,可作为生产应用的参考剂量。
[Abstract]:Transport stress is a kind of complex stress affected by many factors. The state of sheep after transport stress is generally called transport stress syndrome. The occurrence of transport stress also affects the neuroendocrine system, immune function, blood physiological index, blood biochemical index and production performance of the transported sheep. A 14-day stress relief experiment was carried out in small tail Han sheep which was transported over a long distance. Astragalus polysaccharide powder and soluble silver yellow powder were used as experimental drugs. In this experiment, the indexes related to transport stress and the changes of incidence and production performance of small tail Han sheep at different time after long-distance transportation were measured. It is concluded that the experimental drugs have a good relieving effect on the transport stress of the small tail Han sheep and the most suitable quantity of the experimental drugs, which provides the corresponding basis for alleviating the transport stress and reducing the economic loss in the production practice. The results showed that after long distance transportation, the sheep were in the condition of strong transport stress at the beginning of the experiment, and their physiological and biochemical indexes were not within the normal range. As the experiment was carried out, the stress state of the sheep began to ease. In terms of production performance, the intake and weight gain of small tail Han sheep were significantly different between the experimental group and the control group. The ratio of feed to meat in the experiment group was 4.89, which was better than that in the control group (5.80), which indicated that the performance of the sheep in the test group was obviously better than that in the control group. The changes of blood biochemical indexes during the trial period showed that the recovery rate of the relative biochemical indexes of the test group was significantly better than that of the control group, in which creatine kinase was used as the main biochemical index. The contents of lactate dehydrogenase and cortisol changed most significantly. The three clinical indexes showed that the recovery speed of body temperature, pulse and respiratory frequency of the small tail Han sheep in the test group was obviously better than that in the control group, and could recover to the normal level more quickly. The results of blood routine test showed that the total number of white blood cells was higher than the normal value, but the percentage of lymphocyte was lower than the normal value. As the total number of white blood cells began to decrease and the percentage of lymphocytes began to rise, the recovery rate of white blood cells in the test group was obviously better than that in the control group. During the trial period, the incidence of respiratory and digestive tract diseases in sheep was significantly decreased. The incidence of respiratory tract disease and digestive tract disease were 3.26% and 4.59% in the control group, and 1.12% and 2.55% in the optimum dosage group, respectively. Conclusion: the combination of astragalus polysaccharide powder and silver yellow soluble powder can regulate the physiological function and improve the clinical indexes and physiological and biochemical indexes of small tail Han sheep under long distance transportation. At the same time, it can improve the production performance and reduce the incidence, which has good effect on alleviating the transport stress of sheep. The optimum dosage of astragalus polysaccharide powder and silver astragalus powder was 1.0g/ / 1.0g/ / day, which could be used as a reference dosage for production and application.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.74

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