SPF雏鸡感染REV后免疫器官氧化—抗氧化功能的变化

发布时间：2018-05-10 18:00

本文选题：禽网状内皮组织增生病病毒 + SPF雏鸡　；参考：《东北农业大学》2017年硕士论文

【摘要】：禽网状内皮组织增生病(Reticuloendotheliosis,RE)是由禽网状内皮组织增生病病毒(Reticuloendotheliosis virus,REV)引起的免疫抑制性肿瘤病,该病严重危害养禽业的发展。本试验以1日龄SPF雏鸡为研究对象,应用间接ELISA及实时荧光定量PCR方法检测了REV感染雏鸡免疫器官(胸腺、脾脏、法氏囊)过氧化氢(H2O2)、丙二醛(Malondialdehyde,MDA)含量,总抗氧化能力(Total-antioxidante capacity,T-AOC),超氧化物歧化酶(Superoxide dismutase,SOD)活力,过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶1(Glutathione peroxidase 1,GPx1)含量及其mRNA表达的动态变化,旨在从病毒感染后氧化-抗氧化功能变化的角度阐述REV致病机制,为RE的防治提供科学依据。试验结果发现:(1)感染REV后,SPF雏鸡免疫器官H2O2、MDA含量不同程度高于对照雏鸡。其中,胸腺、脾脏、法氏囊H2O2含量分别于感染后第35 d、第28 d、第21 d显著高于对照雏鸡(P0.05);MDA含量分别于感染后第28 d、7 d-28 d、第21 d显著或极显著高于对照雏鸡(P0.05或P0.01)。表明REV感染可导致SPF雏鸡免疫器官氧化物质增多,脂质过氧化反应增强,机体处于氧化应激。(2)感染REV后,SPF雏鸡免疫器官T-AOC、SOD活力不同程度低于对照雏鸡。其中,胸腺、脾脏、法氏囊T-AOC分别于感染后21 d-35 d、28 d-35 d、第35 d显著或极显著低于对照雏鸡(P0.05或P0.01);SOD活力分别于感染后14 d-28 d、14 d-21 d、21 d-35 d显著或极显著低于对照雏鸡(P0.05或P0.01)。表明REV感染可使SPF雏鸡免疫器官抗氧化功能显著降低。(3)感染REV后,SPF雏鸡免疫器官CAT、GPx1含量及基因表达水平不同程度低于对照雏鸡。其中,胸腺、脾脏、法氏囊CAT含量分别于感染后14 d-28 d、28 d-35 d、第21 d显著或极显著低于对照雏鸡(P0.05或P0.01);胸腺、脾脏、法氏囊GPx1含量分别于感染后第28 d、14 d-21 d、第14 d显著或极显著低于对照雏鸡(P0.05或P0.01);胸腺、脾脏CAT mRNA含量分别于感染后21 d-28 d显著或极显著低于对照雏鸡(P0.05或P0.01);胸腺、脾脏、法氏囊GPx1 mRNA含量分别于感染后28 d-35 d、第35 d、第28 d显著低于对照雏鸡(P0.05)。表明REV感染可导致免疫器官CAT、GPx1基因及蛋白表达下降,清除过氧化物能力降低,机体抗氧化酶含量减少,抗氧化功能减弱。(4)感染REV后,SPF雏鸡胸腺、脾脏、法氏囊器官指数于28 d-35 d显著或极显著低于对照雏鸡(P0.05或P0.01)。表明REV感染可使免疫器官器官指数下降,免疫器官萎缩,机体发生免疫抑制。
[Abstract]:Reticuloendotheliosissis-Reticuloendotheliosis-Reticuloendotheliosis virus (Reticuloendotheliosis virus Rev) is a kind of immunosuppressive tumor caused by reticuloendotheliosis virus (Rev), which seriously harms the development of poultry industry. The content of H _ 2O _ 2 H _ 2O _ 2, malondialdehyde (MDA) and malondialdehyde (MDA) in immune organs (thymus, spleen, bursa) of chicks infected with REV were detected by indirect ELISA and real-time fluorescence quantitative PCR. The dynamic changes of total antioxidant capacity, activity of superoxide dismutase (SOD), catalase (catalase), glutathione peroxidase (1(Glutathione peroxidase) 1GPx1) and their mRNA expression were observed. The aim of this study was to elucidate the pathogenetic mechanism of REV from the point of view of the changes of oxidation-antioxidant function after virus infection, and to provide scientific basis for the prevention and treatment of RE. The results showed that the content of MDA in the immune organ of chicken infected with REV was higher than that of the control. The H2O2 content of thymus, spleen and bursa of Fabricius was significantly higher than that of control chicks on the 35th day, the 28th day and the 21st day after infection, respectively. The content of H2O2 in the thymus, spleen and bursa of the control was significantly higher than that of the control chicks on the 28th day, the 7th day and the 21st day, respectively, and was significantly or extremely higher than that of the control chicks on the 35th day, the 28th day, the 21st day, respectively. The results showed that REV infection could lead to the increase of oxidative substances in immune organs and the enhancement of lipid peroxidation in SPF chicks, and the activity of T-AOC- SOD in immune organs of SPF chicks was lower than that of control chicks after REV infection. The T-AOC activity of thymus, spleen and bursa of Fabricius was significantly or extremely lower than that of control chicks on day 35 after infection at 21 d ~ 35 d, respectively. The activity of T-AOC in thymus, spleen and bursa of Fabricius was significantly or extremely lower than that of control chicks at 14 d ~ 28 d ~ (14 d) ~ 21 d ~ (21) d ~ (35) after infection. The results showed that REV infection could significantly decrease the antioxidant function of immune organs of SPF chicks.) after REV infection, the content of SPF GPx1 and the level of gene expression in the immune organs of SPF chicks were lower than those of control chicks. The contents of CAT in thymus, spleen and bursa of Fabricius were significantly or extremely lower than those of control chicks on the 21st day (P 0.05 or P 0.01), and the thymus, spleen and spleen were significantly lower than those of control chicks at 14 d ~ 28 d ~ (28) d ~ (35) after infection. The GPx1 content of bursa of Fabricius was significantly or extremely lower than that of control chicks on day 14 and day 21, respectively, and the CAT mRNA content of thymus and spleen was significantly or very significantly lower than that of control chicks on day 21 and 28, respectively, and the contents of thymus and spleen were significantly or extremely lower than those of control chicks at 21 d to 28 d after infection, and the thymus, spleen, thymus and spleen, respectively. The content of GPx1 mRNA in bursa of Fabricius was significantly lower than that of control chicks at 28 d, 35 d and 28 d after infection, respectively. The results showed that REV infection could decrease the expression of REV GPx1 gene and protein, decrease the ability of scavenging peroxide, decrease the content of antioxidant enzymes, and weaken the antioxidant function of REV.) after infection with REV, the thymus and spleen of REV chicks were infected. The organ index of bursa of Fabricius was significantly or extremely significantly lower than that of control chicks (P0.05 or P0.01) at 28 d ~ 35 d. The results showed that REV infection could decrease the immune organ index, decrease the immune organ atrophy and induce immunosuppression.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31

【参考文献】