安徽地区猪源链球菌分离鉴定及其生物学特性研究

发布时间：2018-05-13 17:00

本文选题：安徽地区 + 猪链球菌　；参考：《安徽农业大学》2015年硕士论文

【摘要】：猪链球菌病是各种链球菌引致猪的疫病的总称,引致猪链球菌病的链球菌种型繁多,不同种型之间生物学特性差异显著,不同地区或时间优势种型亦呈动态过程,这给其防治带来了严重困难。本研究针对2009~2014年源自安徽地区临床病例的猪源链球菌,采用PCR方法鉴定猪链球菌,1、2、7、9型猪链球菌及mrp、ef、sly毒力基因;梅里埃VITEK 2全自动细菌分析仪鉴定链球菌中的非猪链球菌;PFGE(脉冲场凝胶电泳)对优势菌基因分型;K-B纸片扩散法检测优势菌的药物敏感性,旨在了解安徽地区猪源链球菌生物学特性,初步建立安徽地区猪源链球菌PFGE数据库,探讨安徽地区猪源链球菌之间的联系,为安徽地区猪链球菌病致病菌的溯源和分子流行病学研究提供科学依据,对安徽地区猪链球菌病的防治具有重要意义。结果显示:在196株链球菌中猪链球菌112株,占57.1%,为优势菌,其次为肠球菌40株,占20.4%。在112株猪链球菌中:2型44株,占39.3%,为优势血清型;ef、mrp、sly毒力基因携带率分别为39.3%、36.6%、56.3%,ef-/mrp-/sly-(36株、32.1%)为优势毒力基因型,2型猪链球菌ef、mrp、sly毒力基因携带率明显高于猪链球菌携带率,分别为75.0%、52.3%、88.6%,ef+/mrp+/sly+(19株、43.2%)为其优势毒力基因型;共分为56个PFGE基因型,Ps28(18株,16.1%)和Ps27(14株,12.5%)为优势PFGE基因型,且主要为2型猪链球菌;仅对万古霉素、氨苄西林、阿莫西林、氧氟沙星、头孢拉宁敏感率达50.0%以上,对四环素、磺胺甲基异恶唑、链霉素、强力霉素、庆大霉素、阿奇霉素、复方新诺明耐药率达85.0%以上,对青霉素、头孢曲松、头孢噻肟、头孢吡肟、恩诺沙星、替考拉宁耐药趋势明显,多重耐药现象严重,耐8种以上药物的菌株96株,占85.7%。在40株肠球菌中:以屎肠球菌(21株、52.5%)为主;共分为20个PFGE基因型,其中Pe12(9株,22.5%)和Pe7(7株,17.5%)为优势PFGE基因型,且主要为屎肠球菌;仅对万古霉素,替考拉宁敏感率达72.0%以上,对链霉素、复方新诺明、磺胺甲基异恶唑、庆大霉素、头孢吡肟、四环素、阿奇霉素、头孢曲松耐药率达85.0%以上,多重耐药现象严重,耐8种以上药物的菌株38株,占95.0%。结果表明:安徽地区猪源链球菌种型复杂,猪链球菌为优势菌,2型猪链球菌为优势血清型,ef-/mrp-/sly-为优势毒力基因型。肠球菌已成为安徽地区养猪业重要的致病菌。猪链球菌和肠球菌PFGE基因型呈多态性,PFGE基因型与血清型、毒力基因型不呈现相关性,2型猪链球菌、屎肠球菌分别主要源自两个克隆系、且传播过程存在遗传变异。猪链球菌和肠球菌耐药现象严重,多重耐药谱越来越广。
[Abstract]:Streptococcus suis disease (Streptococcus suis) is the general name of the epidemic disease caused by Streptococcus. The Streptococcus suis has a wide variety of streptococcal species, and the biological characteristics of different species differ significantly. The different regional or time dominant species also have a dynamic process. This is a serious difficulty for the prevention and control of the streptococcal disease. This study was based on the clinical cases of Anhui in Anhui. Streptococcus suis, Streptococcus suis, Streptococcus suis, Streptococcus suis 1,2,7,9 and MRP, EF, sly virulence genes were identified by PCR, and non Streptococcus in Streptococcus was identified by the full-automatic bacterial analyzer of VITEK 2; PFGE (pulse field gel electrophoresis) was used to genotyping the dominant bacteria; K-B paper diffusion method was used to detect the drug sensitivity of dominant bacteria. The biological characteristics of Streptococcus suis in Huizhou area were preliminarily established to establish the PFGE database of Streptococcus suis in Anhui area, to explore the relationship between Streptococcus and Streptococcus in Anhui area, and to provide scientific basis for the study of the origin and molecular epidemiology of Streptococcus suis pathogens in Anhui. It is of great significance to the prevention and control of Streptococcus suis disease in Anhui. The results showed that in 196 Streptococcus, 112 strains of Streptococcus suis, 57.1%, dominant and 40 of Enterococcus, accounted for 20.4%. in 112 strains of Streptococcus suis, 2 type 44, 39.3%, and dominant serotype; EF, MRP, sly virulence genes were 39.3%, 36.6%, 56.3%, ef-/mrp-/sly- (36, 32.1%) were dominant virulence genotype, Streptococcus suis EF, MRP The carrying rate of sly virulence gene was significantly higher than that of Streptococcus suis, 75%, 52.3%, 88.6%, and ef+/mrp+/sly+ (19, 43.2%) were the dominant virulence genotypes, which were divided into 56 PFGE genotypes, Ps28 (18, 16.1%) and Ps27 (14, 12.5%) as the dominant PFGE based genotype, and mainly Streptococcus suis, and only vancomycin, ampicillin, Amos The susceptibility rate of forest, ofloxacin and cefpenine was over 50%. The resistance rates of tetracycline, sulfamethoxazole, streptomycin, doxycycline, gentamicin, azithromycin, and compound neomycin were more than 85%. The resistance to penicillin, ceftriaxone, cefotaxime, cefpimoxime, enrofloxacin and teicoplanin was obvious, and multidrug resistance was serious. 96 strains resistant to more than 8 kinds of drugs, accounting for 85.7%. in 40 strains of enterococci: Enterococcus faecium (21, 52.5%), were divided into 20 PFGE genotypes, of which Pe12 (9, 22.5%) and Pe7 (7, 17.5%) were dominant PFGE genotypes and were mainly Enterococcus faecium; only vancomycin, teicoplanin was more than 72%, streptomycin, compound novamine, The drug resistance rate of sulfamethoxazole, gentamicin, cefepime, tetracycline, azithromycin, and ceftriaxone was over 85%, and multiple drug resistance was serious and 38 strains were resistant to more than 8 kinds of drugs. The results of 95.0%. showed that the pig source streptococcal species in Anhui area was complex, Streptococcus suis was dominant, and Streptococcus suis 2 was the dominant serotype, ef-/mrp-/sl Y- is the dominant virulence genotype. Enterococcus has become an important pathogenic bacteria in Anhui pig industry. Streptococcus suis and Enterococcus PFGE genotypes are polymorphic. The PFGE genotype is not associated with serotypes and virulence genotypes. The type 2 Streptococcus suis and Enterococcus faecium are mainly derived from two clone lines, and the transmission process has genetic variation. The drug resistance of cocci and enterococci is serious, and the spectrum of multidrug resistance is increasing.

【学位授予单位】：安徽农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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