内蒙古部分地区致奶牛乳房炎金黄色葡萄球菌和产色葡萄球菌的分子流行病学及耐药性研究

发布时间：2018-05-14 06:44

本文选题：金黄色葡萄球菌 + 产色葡萄球菌　；参考：《中国预防兽医学报》2017年04期

【摘要】：为调查奶牛乳房炎中金黄色葡萄球菌和产色葡萄球菌的流行病学和耐药性情况,本研究对采集自内蒙古部分地区3个规模化奶牛场的330份奶样进行了葡萄球菌的分离、鉴定、耐药表型分析及基因型的检测;采用脉冲场凝胶电泳(PFGE)方法对分离株进行了亲缘性分析,多位点序列分型(MLST)法对金黄色葡萄球菌进行了基因分型分析。结果表明,从330份奶样共分离到14株金黄色葡萄球菌和23株产色葡萄球菌,两者的分离率分别为4.24%(14/330)和6.97%(23/330);PFGE分型共获得8种不同谱型的金黄色葡萄球菌和16种不同谱型的产色葡萄球菌,表明这些分离株中存在克隆传播现象;药敏试验结果显示,两种病原菌对林可霉素、青霉素、苯唑西林和红霉素耐药率较高,分别为66.67%(16/24)、66.67%(16/24)、37.5%(9/24))和25%(6/24);介导青霉素耐药的bla Z基因检出率最高,为54.17%(13/24),其次为介导林可霉素和红霉素耐药的Inu(A)和erm(B)基因,其检出率分别为33.33%(8/24)和20.83%(5/24)。本研究结果为控制该地区奶牛乳房炎的传播、蔓延及临床用药提供了实验依据。
[Abstract]:In order to investigate the epidemiology and drug resistance of Staphylococcus aureus and Staphylococcus chromogens in mastitis of dairy cows 330 milk samples from 3 large scale dairy farms in Inner Mongolia were isolated and identified. The phenotypic analysis and genotypic analysis of drug resistance were carried out, and the genotyping of Staphylococcus aureus was carried out by using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The results showed that 14 strains of Staphylococcus aureus and 23 strains of Staphylococcus aureus were isolated from 330 milk samples. The isolation rates of the two isolates were 4.2414 / 330) and 6.97% / 330% PFGE, respectively. Eight different types of Staphylococcus aureus and 16 different spectral forms of Staphylococcus aureus were obtained, which indicated that there was clonal transmission in these isolates, and the results of drug sensitivity test showed that, The resistance rates of the two pathogens to lincomycin, penicillin, oxacillin and erythromycin were higher (66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.67 / 66.65 / 24) and 256.624% respectively. The detection rate of bla Z gene in penicillin resistance was the highest at 54.17% 132424%, followed by the genes Inuia and Ermbib, which mediated the resistance to lincomycin and erythromycin. The detection rates were 33.33 / 24 and 20.83 / 24, respectively. The results of this study provide experimental basis for controlling the spread, spread and clinical use of mastitis in dairy cows in this area.
【作者单位】：吉林农业大学动物科学技术学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室;内蒙古伊利实业集团股份有限公司;天津市畜牧兽医研究所;
【基金】：国家自然科学基金青年项目(31201862) 内蒙古自治区科技重大专项
【分类号】：S858.23

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