广东省猪伪狂犬病流行病学调查及带毒情况的研究

发布时间：2018-05-14 13:48

本文选题：PRV + PCR　；参考：《河南农业大学》2015年硕士论文

【摘要】：伪狂犬病(Pseudorabies Disease)又被称作奥耶基氏病(Aujeszky’s,AD),是由伪狂犬病毒(Pseudorabies Viurs,PRV)引起的,包括多种家畜和野生动物以发热、奇痒(猪除外)和脑脊髓炎等为主要特征的一种急性、高度接触性的烈性传染病,其中,尤以猪的感染最为常见。目前,猪伪狂犬病已经在全球范围内流行,我国也已有20多个地区先后发生本病,并且开始具有蔓延和上升的趋势。因此,该病已被OIE列为B类动物疫病,我国也将其列为二类动物疫病,并且该病在广东省的普遍存在也已经严重影响到养猪业的健康发展。为了弄清广东省猪伪狂犬病的流行情况,便于做好防控工作,本篇论文从以下几个方面进行了调查和研究:1.广东省猪伪狂犬病的流行病学研究对2014年期间广东省猪伪狂犬病血清和分子流行情况进行了调查和分析。从地域分布看,珠三角地区的PR流行率最高(30.41%),其次是粤西地区(21.14%),粤东(11.31%)、粤北(9.69%)PR流行率相对较低。从时间分布上看,综合野毒抗体阳性率和PRV抗原检出阳性率来看,PRV阳性率较高的月份是3月,4月和12月,多集中在气候相对不稳定的季节。2.广东省猪伪狂犬病毒毒株的分离鉴定及序列分析将广东省分离到的8种PRV分离株的gE、TK基因与国内外的毒株进行核苷酸及氨基酸的序列同源性分析发现,分离毒株的gE、TK基因与15株国内外已发表的代表毒株核苷酸及氨基酸的序列同源性分别为97.3%~99.9%、98.7%~99.9%;遗传进化分析表明,分离毒株与国内近年分离自不同省份的代表毒株亲缘关系较近,与国外分离的代表毒株亲缘关系较远,而g E、TK基因氨基酸主要变异位点分析表明gE和TK基因都有一些位点已经发生突变。本研究的结果表明猪伪狂犬病毒在广东地区部分地区分离毒株已发生变异,为规模化猪场对猪伪狂犬病的净化以及疫苗的选择提供一定参考依据。3.广东省猪伪狂犬病的排毒情况与g E、gB抗体水平关系的研究本实验通过对猪伪狂犬病阳性猪场和阴性猪场不同时间段不同猪群的PRV和gE与gB抗体水平的监测,分析猪群中PRV排毒情况和抗体水平的关系,根据监测结果制定综合防控措施用以更好的遏制猪场内伪狂犬病的流行。
[Abstract]:Pseudorabies disease (Pseudorabies disease), also known as Aujeszkykykysashian, is caused by pseudorabies Viurs (PRV) and includes an acute condition characterized by fever, itching (except pigs) and encephalomyelitis in a variety of domestic animals and wild animals, including, among other things, pseudorabies Viursvera, or pseudorabies, or pseudorabies. High contact infectious disease, especially in pigs. At present, pig pseudorabies has been prevalent all over the world, and it has occurred successively in more than 20 regions in China, and has begun to spread and rise. Therefore, the disease has been classified by OIE as a class B animal epidemic disease, and it is also classified as a second class animal epidemic disease in China. The prevalence of the disease in Guangdong Province has seriously affected the healthy development of pig industry. In order to find out the epidemic situation of pig pseudorabies in Guangdong province and to do the prevention and control work well, this paper has carried on the investigation and the research from the following several aspects. An epidemiological study on swine pseudorabies in Guangdong province was conducted to investigate and analyze the serological and molecular prevalence of pseudorabies in Guangdong province during 2014. In terms of regional distribution, PR prevalence rate was the highest in Pearl River Delta, followed by 21.14 in western Guangdong, 11.31 in eastern Guangdong, and 9.69 in northern Guangdong. From the point of view of time distribution, the positive rate of PRV antibody and PRV antigen were higher in March, April and December, mostly in the season of relatively unstable climate. Isolation, identification and sequence analysis of porcine pseudorabies virus strain in Guangdong Province. The nucleotide and amino acid sequence homology analysis of the gEtoTK gene of 8 PRV isolates isolated from Guangdong Province and domestic and foreign strains were found. The nucleotide sequence and amino acid sequence homology of the isolated strain was 97.3 ~ 99.9 ~ 99.70.The genetic evolution analysis showed that the relationship between the isolated virus strain and the representative virus strain isolated from different provinces in recent years was close to that of the representative virus strain isolated from different provinces in China, the genetic evolution analysis showed that the genetic evolution of the isolated strain was similar to that of the representative virus strain isolated from different provinces in China in recent years. The analysis of the main amino acid mutation sites of the g EtoTK gene showed that both GE and TK genes had some mutations. The results of this study indicated that the isolates of porcine pseudorabies virus had mutated in some areas of Guangdong province, which provided a certain reference basis for the purification of pig pseudorabies and the selection of vaccine in large-scale pig farms. Study on the relationship between the detoxification of porcine pseudorabies in Guangdong Province and the level of g E GB antibody the levels of PRV, GE and GB antibodies in different breeds of pig farms with and without pseudorabies in different periods were monitored in this experiment. Based on the analysis of the relationship between PRV detoxification and antibody level in swine herd, comprehensive prevention and control measures were formulated to better control the prevalence of pseudorabies in pig farms.
【学位授予单位】：河南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

【相似文献】