番鸭呼肠孤病毒人工感染雏番鸭肝的蛋白质组学分析

发布时间：2018-05-14 22:21

本文选题：番鸭呼肠孤病毒 + 人工感染　；参考：《畜牧兽医学报》2017年10期

【摘要】：应用蛋白质组学技术研究人工感染番鸭呼肠孤病毒(MDRV)的番鸭肝和健康对照番鸭肝的蛋白质组差异,为深入研究MDRV的致病机制奠定理论基础。通过二维双向凝胶电泳技术和MALDI-TOF-TOF质谱仪分析获取MDRV感染组与健康对照组之间的差异表达蛋白质,并运用蛋白质搜索鉴定软件Mascot 2.3.02鉴定蛋白质。结果共鉴定出38个差异表达蛋白质。比较感染组和健康对照组结果,感染组番鸭肝中有10个表达下调的蛋白质和17个表达上调的蛋白质,7个表达蛋白质仅出现在健康对照组,4个表达蛋白质仅出现在感染组。MDRV感染组的番鸭肝有肝细胞、神经细胞和肌肉细胞受损现象,同时存在细胞修复机制。差异蛋白质组学为MDRV分子发病机制的分析提供了一个新途径。
[Abstract]:The proteome differences between muscovy duck liver infected with muscovy duck reovirus (MDRV) and healthy muscovy duck liver were studied by using proteomics technique, which laid a theoretical foundation for further study on the pathogenesis of MDRV. The differentially expressed proteins between MDRV infected group and healthy control group were obtained by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometry, and protein identification software Mascot 2.3.02 was used to identify the protein. Results 38 differentially expressed proteins were identified. The results of infection group and healthy control group were compared, There were 10 down-regulated proteins and 17 up-regulated proteins in the infected muscovy duck liver, 7 expressed proteins only appeared in the healthy control group, and 4 expressed proteins only appeared in the muscovy duck liver of the infected group. MDRV infected muscovy duck liver had hepatocytes. Nerve cells and muscle cells are damaged, and there is a mechanism of cell repair at the same time. Differential proteomics provides a new approach for the analysis of the pathogenesis of MDRV molecules.
【作者单位】：安徽省广德县畜牧兽医水产局;福建省农业科学院畜牧兽医研究所;福建省畜禽疫病防治工程技术研究中心;福建省农业科学院生物技术研究所;
【基金】：福建省自然科学基金(2014J01106) 福建省重大研发平台(2014N2003-7) 国家自然科学基金(31172334)
【分类号】：S858.32

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