6个猪品种CD53基因中结构变异SV184分布频率的研究

发布时间：2018-05-14 23:32

本文选题：猪品种 + CD基因　；参考：《中国畜牧兽医》2017年04期

【摘要】：基于香猪全基因组重测序数据,在白细胞表面抗原CD53基因中筛选到一个结构变异(structure variant,SV)184,为了探索不同猪品种间此结构变异是否存在多态性变化,本试验选择香猪、大白猪、糯谷猪、柯乐猪、江口萝卜猪和荣昌猪作为试验动物,采用PCR方法对猪群中SV184的分布频率进行比较研究,应用RT-PCR技术,分析CD53基因的原初转录本序列结构,研究SV184对CD53基因的转录是否有直接影响。结果显示,6个猪品种中SV184的分布频率存在明显差异,香猪以VN基因型为主,其他5个猪品种是NN基因型占优势;计算两种等位基因的频率,香猪以缺失的V等位基因为主,其他5个猪品种以正常的N等位基因为主。选择3种基因型个体的血液样本,逆转录后分析CD53基因的原初转录本的序列,从中检测到缺失型和不缺失的两种转录本形式,提示SV184对CD53基因的转录无直接影响。SV184可作为分子标记用于区分香猪和其他猪品种。
[Abstract]:Based on the whole genome resequencing data of Xiang pig, a structural variation structure variant CD53 gene was screened from white blood cell surface antigen (WSA) gene. In order to explore whether there is a polymorphic variation in this variation among different pig breeds, Xiang pig and big white pig were selected in this experiment. As experimental animals, the distribution frequency of SV184 in pigs was studied by PCR method, and the original transcription sequence structure of CD53 gene was analyzed by RT-PCR technique. To study whether SV184 has a direct effect on the transcription of CD53 gene. The results showed that there was significant difference in the distribution frequency of SV184 among the six pig breeds, the VN genotype was dominant in Xiang pig, the NN genotype was dominant in the other five pig breeds, the frequency of the two alleles was calculated, and the missing V allele was the main allele in Xiang pig. Normal N alleles were the main alleles in the other 5 pig breeds. The original transcripts of CD53 gene were sequenced by reverse transcription from blood samples of three genotypes, and the deletion and non-deletion transcripts were detected. These results suggest that SV184 has no direct effect on the transcription of CD53 gene. SV184 can be used as a molecular marker to distinguish Xiang pig from other pig breeds.
【作者单位】：贵州大学动物科学学院;贵州大学农业工程研究院;
【基金】：国家高技术研究发展计划(863计划)课题(2013AA102503) 贵州省农业攻关项目(黔科合NY[2013]3073号) 贵州省“百”层次创新型人才项目(黔科合人才2016-4012号) 国家自然科学基金(31672390)
【分类号】：S828

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