年龄、部位对新疆驴皮中Ⅰ型胶原蛋白表达影响的研究

发布时间：2018-05-15 15:37

本文选题：驴皮 + 胶原蛋白　；参考：《中国畜牧杂志》2017年12期

【摘要】：本研究旨在探究不同年龄、不同部位新疆驴皮中Ⅰ型胶原蛋白2个亚基基因(COL1A1、COL1A2)mRNA的表达量及其蛋白含量的差异,确定宰驴取皮的年龄段,以提高驴皮的利用率。对不同年龄段新疆驴背部皮肤组织,应用RT-qPCR、Western blotting技术检测胶原蛋白基因的表达量及其蛋白含量;对新疆驴不同部位皮肤进行HE染色切片与RT-qPCR技术分析。结果表明:6~7岁年龄段的驴皮中COL1A1、COL1A2基因的表达量及其蛋白含量显著高于其他各年龄段(P0.05)。从组织切片上可以看到,驴皮背部与颈部的胶原纤维密集且多,而脸部的疏松且少,腿部和腹部居中;这与其胶原蛋白基因RT-qPCR的结果相一致。综上,6~7岁年龄段的新疆驴背部皮肤中,胶原蛋白基因的表达量及含量最高,故此年龄段可作为宰驴取皮的最佳年龄段。
[Abstract]:The purpose of this study was to investigate the difference of mRNA expression and protein content of two subunit genes of collagen type I in Xinjiang donkey skin of different ages and different parts, and to determine the age of skin removal from donkey slaughtering in order to improve the utilization rate of donkey skin. The expression of collagen gene and its protein content were detected by RT-qPCR- Western blotting in the dorsal skin of Xinjiang donkey at different ages, and HE staining and RT-qPCR technique were used to analyze the different parts of the skin of Xinjiang donkey. The results showed that the expression of COL1A1A2 gene and its protein content in the donkey skin of 7 years old were significantly higher than those of other age groups (P0.05A). It can be seen from tissue section that the collagen fibers in the back and neck of the donkey skin are dense and much, but the face is loose and less, and the legs and abdomen are in the middle, which is consistent with the result of RT-qPCR. The expression and content of collagen gene were the highest in the back skin of Xinjiang donkeys aged 6 to 7 years old, so the age group could be regarded as the best age for the removal of donkey skin.
【作者单位】：石河子大学动物科学学院;塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室;中国农业大学动物科技学院;
【基金】：国家自然科学基金项目(31360530、31260531) 兵团塔里木畜牧科技重点实验室项目(HS201503)
【分类号】：S822

【相似文献】