焦麦芽、谷氨酰胺对断奶仔猪肠道消化及屏障功能的影响

发布时间：2018-05-16 12:21

本文选题：焦麦芽 + 谷氨酰胺　；参考：《山西农业大学》2016年硕士论文

【摘要】：本研究旨在比较焦麦芽和谷氨酰胺(Gln)在改善断奶仔猪肠道消化功能及肠道屏障功能上的差异。试验选取21d断奶的杜长白仔猪48头,随机分为4组(每组3个重复,每重复4头猪),分别接受基础日粮(对照组)、1%焦麦芽(焦麦芽组)、1%Gln(Gln组)、1%焦麦芽+1%Gln(复合组)四种处理,分别在试验第7d、14d进行空腹屠宰采样,利用酶学、组织与电镜学、免疫组化技术、酶联免疫技术,分别测定了仔猪肠道消化酶活性、肠道形态结构、肠组织IgA分泌量及细胞因子IL-2、IFN-γ、IL-1β的含量。试验结果如下：1)焦麦芽、Gln均能有效提高十二指肠和空肠胰蛋白酶、脂肪酶及淀粉酶活性,其中,焦麦芽组与Gln组相比,焦麦芽显著提高了试验第7d十二指肠胰蛋白酶、十二指肠以及空肠脂肪酶的活性,同时也显著提高了试验第14d十二指肠胰蛋白酶、脂肪酶以及空肠胰蛋白酶活性(P0.05)；而Gln显著提高试验第14d肠道淀粉酶活性(P0.05)；2)焦麦芽、G1n均能显著提高肠道VH与V/C(P0.05),其中,焦麦芽组与Gln组相比,G1n显著提高了试验第7d、14d十二指肠VH(P0.05),焦麦芽显著提高了试验第7d、14d回肠VH,而在空肠VH上,两者差异不显著(P0.05)；3)焦麦芽、G1n均能提高肠道IgA蛋白的分泌量及免疫细胞因子的含量。其中,焦麦芽组与Gln组相比,Gln显著提高了试验第7d十二指肠IgA蛋白的分泌量(P0.05),而在试验第14d,两者差异不显著(P0.05)；Gln提高了试验第7 d、14 d十二指肠IL-2、IL-1β含量,焦麦芽提高了试验第7d空肠和回肠及试验第14d空肠IL-2、IFN-γ、IL-1β含量。4)复合组与焦麦芽组、Gln组相比,试验7 d,复合组空肠胰蛋白酶活性、十二指肠和空肠淀粉酶活性、肠道V/C及空肠IgA蛋白的分泌量与两组相比差异不显著(P0.05),试验14d,复合组十二指肠和空肠胰蛋白酶活性、十二指肠脂肪酶活性、空肠和回肠的VH、V/C及肠道IgA蛋白分泌量与两组相比差异不显著(P0.05)；试验期内,复合组空肠和回肠IL-2、十二指肠和空肠IFN-7、十二指肠和回肠IL-1p与焦麦芽组、Gln组相比差异均不显著(P0.05)。结论：在改善断奶仔猪肠道消化功能上焦麦芽优于Gln；焦麦芽与Gln均能改善断奶仔猪的肠道屏障功能,但两者作用位点不同,Gln主要作用于十二指肠,焦麦芽主要作用于空肠和回肠。
[Abstract]:The purpose of this study was to compare the difference between pyromalt and glutamine Gln in improving intestinal digestive function and intestinal barrier function in weaned piglets. Forty-eight DU Changbai piglets weaned for 21 days were randomly divided into 4 groups (3 repeats in each group and 4 pigs per repeats in each group) and were treated with basic diet (control group 1% pyromalt). Fasting slaughtering samples were collected on the 7th day of the experiment. The digestive enzyme activity and intestinal morphology of piglets were determined by enzymology, tissue and electron microscopy, immunohistochemistry and enzyme-linked immunosorbent assay (Elisa). The secretion of IgA and the content of IL-2 IFN- 纬-1 尾 in intestinal tissue. The results were as follows: (1) GLN could effectively increase the activities of trypsin, lipase and amylase in duodenum and jejunum. Compared with Gln group, pyromalt significantly increased duodenal trypsin on the 7th day. The activity of lipase in duodenum and jejunum was also significantly increased on the 14th day of the experiment. Lipase and jejunal trypsin activity (P0.05N), while Gln significantly increased intestinal amylase activity (P0.05G1n) on the 14th day of the experiment. Compared with the Gln group, the G1n of the pyromalt group increased significantly on the 7th day, and the duodenal VHH P0.05 on the 14th day, and the ileal VH on the 7th day after the treatment, but on the jejunum VH, the G1n of the pyromalt group was significantly higher than that of the Gln group, and that of the coke malt group was significantly higher than that of the Gln group. There was no significant difference between the two groups (P0.05) G1n could increase the secretion of IgA protein and the content of immune cytokines in intestinal tract. Compared with Gln group, the secretion of IgA protein in duodenum of pyromalt group was significantly higher than that of Gln group on the 7th day, but on the 14th day, there was no significant difference between the two groups. The contents of IL-2IFN- 纬 -IL-1 尾 in jejunum and ileum on day 7 and in jejunum on day 14 were increased in pyromalt group. Compared with GLN group, the activity of trypsin and amylase in duodenum and jejunum were increased in the compound group on the 7th day, compared with the GLN group in the scorched malt group on the 7th day, and the activity of amylase in duodenum and jejunum in the combined group on the 7th day. There was no significant difference in the secretion of intestinal V / C and jejunum IgA protein between the two groups (P 0.05). The duodenal and jejunum trypsin activity and the duodenal lipase activity in the compound group were 14 days after the experiment. There was no significant difference in the secretion of VHV / C and IgA protein in jejunum and ileum between the two groups (P 0.05), but there was no significant difference in IL-2, IFN-7, IL-1p in duodenum and jejunum between the two groups during the trial period (P 0.05). Conclusion: in improving intestinal digestive function of weaning piglets, pyromalt and Gln can improve intestinal barrier function of weaned piglets, but different sites of Gluten mainly act on duodenum. Pyromalt mainly acts on jejunum and ileum.
【学位授予单位】：山西农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5

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