硒缺乏对雏鸡肠道菌群及肠上皮细胞黏附性的影响

发布时间：2018-05-17 03:04

本文选题：硒缺乏 + 肠道菌群　；参考：《黑龙江畜牧兽医》2017年07期

【摘要】：为了研究硒缺乏对雏鸡肠道菌群和细菌对肠上皮细胞黏附性的影响,为揭示硒与肠道微生态之间的关系以及临床有效防治雏鸡硒缺乏病提供试验依据,试验选取160只1日龄SPF三黄肉仔鸡,平均分为对照组(饲喂全价饲料)和试验组(饲喂低硒饲料),记录雏鸡生长性能及疾病情况,分别于试验期第10,20,25,30天测定血液和肠黏膜硒含量;采集肠内容物,检测大肠杆菌、葡萄球菌和双歧杆菌等肠道菌群的密集度;制备肠黏膜上皮细胞悬液,检测大肠杆菌和双歧杆菌对肠道黏膜上皮细胞的黏附率。结果表明:与对照组相比,试验组雏鸡十二指肠、空肠、回肠和盲肠中大肠杆菌、沙门氏菌、肠球菌、葡萄球菌及酵母菌数量增高,双歧杆菌和乳酸杆菌数量降低;大肠杆菌对肠道黏膜上皮细胞的黏附率增高,双歧杆菌的黏附率降低。说明雏鸡硒缺乏后肠道菌群失调,有益菌对肠道细胞的黏附性降低,肠道微生态平衡被破坏。
[Abstract]:In order to study the effect of selenium deficiency on intestinal flora and adhesion of bacteria to intestinal epithelial cells in chicks, the relationship between selenium and intestinal microecology and clinical effective prevention and treatment of selenium deficiency in chicks were studied. One hundred and sixty 1-day-old SPF tri-yellow broilers were divided into two groups: the control group (fed with full price feed) and the experimental group (fed with low selenium diet). The growth performance and disease of the chicks were recorded. The selenium content in blood and intestinal mucosa was determined on the 10th day of the trial period, and the intestinal mucosal selenium was collected to detect the concentration of intestinal flora, such as Escherichia coli, Staphylococcus and Bifidobacterium, and to prepare the intestinal mucosal epithelial cell suspension. The adhesion rate of Escherichia coli and Bifidobacterium to intestinal mucosal epithelial cells was determined. The results showed that the number of Escherichia coli, Salmonella, Enterococcus, Staphylococcus and yeast in duodenum, jejunum, ileum and cecum in the experimental group was higher than that in the control group, but the number of Bifidobacterium and Lactobacillus was decreased. The adhesion rate of Escherichia coli to intestinal mucosal epithelial cells was higher than that of Bifidobacterium. The results showed that the intestinal flora of chicks was disordered after selenium deficiency, the adhesion of beneficial bacteria to intestinal cells was decreased, and the intestinal microecological balance was destroyed.
【作者单位】：黑龙江八一农垦大学动物科技学院;
【基金】：国家自然科学基金项目(31272624) 大庆市指导性科技计划项目(zd-2016-094)
【分类号】：S831

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