抗菌中药筛选思路的解析及实践

发布时间：2018-05-17 05:20

本文选题：抗菌中药 + 李斯特菌病　；参考：《中国兽药杂志》2016年02期

【摘要】：为研究防治细菌病的中药方剂及有效成分,解析了抗菌中药的筛选思路,提出了运用传统中兽医整体观念、辨证论治的理论基础进行中药筛选。以治疗动物李斯特菌病为例,根据小鼠动物模型(体内模型)的临床症状和中兽医辨证论治理论,配伍组方筛选出钩藤牛角地黄汤为治疗方剂;结合李斯特菌的致病机理,以微血管内皮细胞为基础建立细胞损伤模型(体外模型),进一步阐释组方中各单味中药及单一药物成分的作用机理,即传统中药是通过改善、调节机体细胞功能缓解细胞损伤而非如抗生素一样直接杀灭细菌。中兽药创新研究务必在传统中兽医的理论指导下选择药物,利用中药保护和调理微血管内皮细胞的形态和功能,阻断细菌毒素对微血管内皮细胞的损伤作用,为中药方剂及其有效成分治疗细菌性疾病提供思路。
[Abstract]:In order to study the prescription and effective components of traditional Chinese medicine for the prevention and treatment of bacterial diseases, this paper analyzes the screening idea of antibacterial Chinese medicine, and puts forward the selection of traditional Chinese medicine based on the whole concept of veterinary medicine and the theoretical basis of syndrome differentiation and treatment. Taking the treatment of animal Listeria as an example, according to the clinical symptoms of mouse animal model (model in vivo) and the theory of differentiation and treatment of traditional Chinese medicine and veterinarian, the prescription was used to screen Gouteng Niujiao Rehmannia decoction as the therapeutic prescription, and the pathogenic mechanism of Listeria was combined. The model of cell injury was established on the basis of microvascular endothelial cells (model in vitro) to further explain the action mechanism of each single Chinese medicine and the single drug component in the prescription, that is, the traditional Chinese medicine is through improvement, Regulate cell function to alleviate cell damage rather than kill bacteria as directly as antibiotics. Under the guidance of traditional veterinary medicine theory, Chinese veterinary medicine innovation research must select drugs, protect and regulate the morphology and function of microvascular endothelial cells by using Chinese medicine, and block the damage effect of bacterial toxin on microvascular endothelial cells. To provide ideas for the treatment of bacterial diseases with traditional Chinese medicine and its active components.
【作者单位】：中国兽医药品监察所;北京农学院动物科学技术学院;中国农业大学动物医学院;
【分类号】：S853

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