腹泻羔羊与健康羔羊肠道微生物群落分析

发布时间：2018-05-17 08:36

本文选题：滩寒杂交羊 + 羔羊　；参考：《动物医学进展》2017年12期

【摘要】：羔羊腹泻与其肠道菌群的种类及比例密切关系。为了探索肠道菌群结构在腹泻病程的变化,采集同样饲养条件下20份1月龄~3月龄滩寒杂交羊粪便样本,通过Miseq测序技术并结合多变量统计学方法检测细菌16SrRNA基因V4-V5区的多样性,通过数据分析,比较了腹泻羊粪便与正常羊粪便中微生物的类别及数量。从门的水平分析,腹泻羔羊肠道中变形菌门显著高于健康羔羊。发现拟杆菌门、蓝细菌门、黏胶球形菌门和无壁细菌门在腹泻羔羊中的含量显著低于健康羔羊。从科水平分析2个样本优势菌具有显著差异,其中腹泻羔羊肠道放线菌乳杆菌科显著高于健康羔羊,而瘤胃球菌科显著低于健康羔羊。非优势菌也存在显著差异,如拟杆菌科、柔膜菌科和理研菌科。比较了滩寒杂交羊健康羔羊及腹泻羔羊肠道微生物的差异,发现其微生物丰度及优势微生物都具有显著的差异,因此,腹泻羔羊中的肠道微生物差异可能是羔羊腹泻的重要因素之一,研究结果为羔羊腹泻的研究及治疗提供了理论依据。
[Abstract]:Lamb diarrhea is closely related to the species and proportion of intestinal flora. In order to explore the changes of intestinal flora structure in the course of diarrhea, 20 feces samples of 1-month-old and 3-month-old cold crossbred sheep were collected under the same feeding conditions. The diversity of the V4-V5 region of bacterial 16SrRNA gene was detected by Miseq sequencing and multivariate statistics. The types and quantities of microbes in diarrhea sheep feces and normal sheep feces were compared by data analysis. The level of phylum in intestinal tract of diarrhea lamb was significantly higher than that of healthy lamb. It was found that the contents of Bacteroides, Cyanobacterium, Viscose and Wall-free bacteria in diarrhea lambs were significantly lower than those in healthy lambs. There were significant differences in the dominant bacteria between the two samples from the family level. The intestinal actinomycetes of diarrhea lambs was significantly higher than that of healthy lambs, while the ruminal coccus family was significantly lower than that of healthy lambs. There were also significant differences in nondominant bacteria, such as Bacteroides, Fleuromycetes, and Lycophagidae. The differences of intestinal microbes between healthy lambs and diarrhea lambs were compared. It was found that there were significant differences in microbial abundance and dominant microorganisms. The differences of intestinal microbes in lambs with diarrhea may be one of the important factors of lamb diarrhea. The results provide a theoretical basis for the study and treatment of lamb diarrhea.
【作者单位】：宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室;宁夏农林科学院动物科学研究所;
【基金】：宁夏自治区留学人员创新创业择优资助项目(宁人社函2014第486号) 宁夏大学人才引进科研启动基金宁夏自然科学基金项目(NZ14017) 宁夏农林科学院先导基金项目(NKYJ-14-10)
【分类号】：S852.6

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