白羽王鸽GHR、MSTN基因多态性与生长性能和屠宰性能的关联性分析

发布时间：2018-05-18 04:15

本文选题：白羽王鸽 + GHR　；参考：《南京农业大学》2015年硕士论文

【摘要】：生长性状和屠宰性状是衡量动物生产性能的两个重要指标。白羽王鸽作为我国优异鸽种资源,深受广大养殖户的喜欢。本研究首先对白羽王鸽7个体尺指标和7个屠宰指标进行测定,并对各指标进行了相关性分析,并选择与生长发育密切相关的GHR基因和MSTN基因为研究对象,通过直接测序的方法扫描筛选其SNP位点(Single nucleotide polymorphism site,SNPs),分析白羽王鸽GHR、MSTN基因的单核苷酸多态性与生长性状之间的相关性,为白羽王鸽分子标记辅助育种实践提供理论依据。论文的主要研究结果和结论如下:(1)对白羽王鸽7个体尺指标和7个屠宰指标进行测定,并对各指标进行了相关性分析。结果表明,相关性始终存在于体重、体尺与屠宰性状之间:体重与除骨盆宽之外的其余6个体尺指标(体斜长、龙骨长、胸深、胸宽、胫长、胫围)相关性均为正相关,且相关性显著或极显著(P0.01)。对白羽王鸽体重与体斜长、胸深、龙骨长、骨盆宽,屠体重与体斜长、胸深、龙骨长、骨盆宽,全净膛重与体斜长、龙骨长,胸肌重与体斜长、龙骨长、骨盆宽、胫长,腿肌重与体斜长、胫围,腹肌重与体斜长、胸宽分别建立了回归模型,模型拟合度F检验均达显著或极显著水平。(2)采用PCR-直接测序的方法对白羽王鸽GHR、MSTN的SNP位点进行扫描,结果在GHR基因中发现6个突变位点,分别为C264T、G410A、C992T、T1060C、G1160T、T1197A,在MSTN基因检测到1个突变位点,位于C114T处。由于碱基的突变,其中 C264T、C992T、T1060C、G1160T、T1197A 和 C114T 共 6 处突变位点改变了编码的氛基酸种类。(3)运用最小二乘法分析表明,GHR基因C264T位点的TT基因型个体的骨盆宽度极显著低于CT和CC基因型个体(P0.01);GHR基因G410A位点的GG基因型个体的出生重量显著高于GA基因型个体(P0.05);在胫围性状上,GHR基因C992T位点的TT基因型CC基因型CT基因型,且差异达到显著水平(P0.05);GHR基因T1060C位点的不同基因型个体在各性状上均未达到差异显著性水平;GHR基因G1160T位点的GT基因型个体的胫长长度显著性高于GG基因型个体(P0.05);GHR基因T1197A位点的TA基因型个体和TT基因型个体的骨盆宽度极显著高于AA基因型个体(P0.01)。(4)运用最小二乘法分析表明,MSTN基因C114T位点处CT基因型个体腹脂重显著高于CC基因型个体(P0.05)。
[Abstract]:Growth traits and slaughter traits are two important indexes to measure the performance of animals. White feather pigeon as an excellent pigeon species resources, by the majority of farmers like. In this study, the seven individual size indexes and seven slaughtering indexes of white feather pigeon were measured, and the correlation of each index was analyzed. The GHR gene and MSTN gene, which were closely related to growth and development, were selected as the research objects. Single nucleotide polymorphism SNPs were screened by direct sequencing, and the correlation between single nucleotide polymorphisms of GHR-MSTN gene and growth traits was analyzed in order to provide theoretical basis for molecular marker-assisted breeding in white feather pigeon. The main results and conclusions of this paper are as follows: (1) the indexes of 7 individual scales and 7 slaughtering indexes of white feather pigeon were measured, and the correlation of each index was analyzed. The results showed that the correlation was always between body weight, body size and slaughter traits. There was a positive correlation between body weight and the other 6 individual measures except pelvis width (body oblique length, keel length, chest depth, chest width, tibial length, tibial circumference). The correlation was significant or extremely significant (P 0.01). The weight and body length of white feather pigeon, chest depth, keel length, pelvis width, butcher weight and body oblique length, chest depth, keel length, pelvic width, full bore weight and body oblique length, keel length, chest muscle weight and body oblique length, keel length, pelvis width, shank length, The regression models of leg muscle weight and body oblique length, tibial circumference, abdominal muscle weight and body oblique length, chest width were established respectively. The fit F test of the model was significant or extremely significant. The SNP site of GHRMSTN was scanned by PCR-direct sequencing. Results six mutation sites were found in the GHR gene, namely C264TG410AnC992T992 T1060CG1160TnT1197A, and one mutation locus was detected in the MSTN gene, which was located at C114T. Because of the mutation of the base, Among them, C264TX C992T1060CG1160TT1197A and C114T mutation sites changed the type of enalic acid.) the results of least square analysis showed that the pelvic width of TT genotype individuals with C264T locus of GHR gene was significantly lower than that of individuals with CT and CC genotypes (P0.01GHR). The results showed that the pelvis width of TT genotype individuals with GHR gene C264T loci was significantly lower than that of individuals with CT and CC genotypes. The birth weight of GG genotype individuals at G410A locus was significantly higher than that of GA genotype individuals (P 0.05). The tibial length of GT genotype individuals with GHR gene G1160T locus was significantly higher than that of GG genotype individuals with different genotype T1060C loci. The tibial length of GT genotype individuals with GHR gene G1160T locus was significantly higher than that with GG genotype individuals. Because the pelvis width of TA genotype individuals and TT genotype individuals at T1197A locus was significantly higher than that of AA genotype individuals (P0.01U. 4) least square analysis showed that the abdominal fat weight of CT genotype individuals at C114T locus of MSTN gene was significantly higher than that of CC genotype individuals.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S836

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