禽沙门菌分离株耐药性及Ⅰ类整合子的检测

发布时间：2018-05-19 16:58

本文选题：家禽 + 沙门菌　；参考：《中国兽医科学》2017年02期

【摘要】：为了分析禽沙门菌临床分禽株出现多重耐药的原因,采用Kirby-Bauer纸片扩散法对61株禽沙门菌分离株进行药敏试验筛选,通过PCR方法检测59株多重耐药沙门菌的Ⅰ类整合子的5’-CS端、3’-CS端和可变区基因并进行测序分析。药敏试验结果显示,所分离的禽沙门菌对青霉素、苯唑西林、利福平和万古霉素的耐药率均达到了100%,但对美福仙、诺氟沙星、阿米卡星、卡那霉素、氯霉素、氟苯尼考相对敏感;96.72%沙门菌能耐受6种及其以上抗菌药物,说明沙门菌的多重耐药性比例高。通过PCR及测序发现,5株禽沙门菌(8.5%)中检测到Ⅰ类整合子基因盒,包括4株雏沙门菌和1株肠炎沙门菌。序列分析结果显示,Ⅰ类整合子基因盒的核苷酸同源性达到100%;通过NCBI的BLAST分析发现,Ⅰ类整合子基因盒内含有dfr17和aad A5基因。因此,尽管禽沙门菌分离株的多重耐药性比例高,但是仅有少数菌株的耐药性与Ⅰ类整合子相关。
[Abstract]:In order to analyze the causes of multidrug resistance in clinical isolates of Salmonella, 61 isolates of Salmonella were screened by Kirby-Bauer disk diffusion test. The 5'-CS terminal and variable region genes of class I integron of 59 multidrug resistant Salmonella strains were detected by PCR and sequenced. The results of drug sensitivity test showed that the resistant rates of the isolated avian Salmonella to penicillin, oxacillin, rifampicin and vancomycin were 100, but to methoxifen, norfloxacin, amikacin, kanamycin, chloramphenicol, The relative sensitivity of florfenicol was 96.72%. Salmonella could tolerate 6 or more antimicrobial agents, which indicated that the rate of multidrug resistance of Salmonella was high. Class I integron gene cassette was detected in 5 strains of Salmonella salmonella by PCR and sequencing, including 4 strains of Salmonella chicks and 1 strain of Salmonella enteritidis. Sequence analysis showed that the nucleotide homology of class I integron gene cassette was 100, and the dfr17 and aad A5 genes were found in class I integron gene cassette by BLAST analysis of NCBI. Therefore, although the proportion of multidrug resistance of Salmonella strains was high, only a few strains were associated with class I integron.
【作者单位】：扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：国家自然科学基金项目(31572530) 公益行业(农业)科研专项(201303044) 江苏高校优势学科建设工程项目江苏省家禽疫病防控工程技术研究中心项目(BM2013450)
【分类号】：S852.61

【相似文献】