河南省2014-2015年猪流行性腹泻病毒流行株遗传进化分析

发布时间：2018-05-21 15:58

本文选题：猪流行性腹泻病毒 + 基因变异　；参考：《中国兽医学报》2017年06期

【摘要】：为监测和探究河南省猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)流行株变异情况,本试验对15株2014-2015年间河南省PEDV流行株S基因进行克隆及全序列测定,与河南省2011-2013年流行株以及国内、外代表毒株进行同源性及遗传变异分析,并对这些毒株S基因的N-糖基化位点及跨膜螺旋区域进行预测。结果表明:本试验得到的15株毒株之间S基因核苷酸同源性为97.0%~99.8%,氨基酸同源性为97.0%~99.5%;与2011-2013年河南流行株核苷酸同源性为97.1%~99.0%,氨基酸同源性为97.2%~98.8%,与其他地区流行毒株核苷酸同源性为92.2%~99.0%,氨基酸同源性为91.9%~99.0%;与经典毒株相比,S1区域N端存在15bp的插入和6bp的缺失,核苷酸同源性为91.7%~93.6%,氨基酸同源性为92.0%~93.6%。系统发育建树结果显示目前的流行毒株与经典毒株处于不同的2个群,流行毒株分处不同亚群。对N-糖基化位点及跨膜螺旋区域的预测得知2014-2015年河南省PEDV与经典株相比出现变异,与2010年暴发时相比存在部分突变,毒株之间存在部分差异,研制出有效的针对流行毒的疫苗依然是目前防控该病的重中之重。
[Abstract]:In order to monitor and investigate the variation of S gene of porcine epidemic diarrhea virus in Henan Province, the S gene of 15 epidemic strains of PEDV in Henan Province from 2014 to 2015 were cloned and sequenced, and compared with those in Henan Province from 2011 to 2013 and in China. The homology and genetic variation of S gene were analyzed and the N-glycosylation sites and transmembrane helical regions of S gene were predicted. The results showed that the nucleotide homology of S gene and amino acid homology among the 15 strains were 97.0 ~ 99.8 and 97.0 ~ 99.5.The nucleotide homology and amino acid homology of the 15 strains were 97.1and 98.8 respectively, and the nucleotide homology and amino acid homology were 97.1and 98.8, respectively, and the nucleotide homology was 97.1%, and the amino acid homology was 98.8% with that of other endemic strains in Henan from 2011 to 2013. The homology of glycoside and amino acid was 92.2% and 91.9%, respectively. Compared with the classical strain, there was 15bp insertion and 6bp deletion in S _ 1 region. The homology of nucleotide and amino acid was 91.7 and 93.6, respectively. Phylogenetic results showed that the current epidemic strains and classical strains were in two different groups, and the epidemic strains had different subgroups. The prediction of N-glycosylation site and transmembrane helical region showed that the variation of PEDV in Henan Province in 2014-2015 was compared with that of classical strains, and there were some mutations and some differences among strains compared with the outbreak in 2010. The development of an effective vaccine against the epidemic is still a top priority in the prevention and control of the disease.
【作者单位】：河南农业大学牧医工程学院;
【基金】：国家自然科学基金资助项目(31272567) 河南省高校创新人才支持计划资助项目(14HASTIT022) 河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)
【分类号】：S852.651

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