马急性蹄叶炎血液中血管活性物及炎性因子变化的研究

发布时间：2018-05-23 11:54

本文选题：马 + 蹄叶炎　；参考：《东北农业大学》2015年硕士论文

【摘要】：运动系统疾病是对马属动物危害极大的一类疾病,蹄叶炎又是运动系统疾病中最常见的疾病。基于此,本实验研究马低聚果糖(OF)急性蹄叶炎模型血液循环中脂多糖(LPS)、脂联素(ADPN)、胰岛素、血糖、白细胞介素-6(IL-6)、p38丝裂原活化蛋白激酶(p38MAPK)、5-羟色胺(5-HT)、内皮素-1(ET-1)、血栓素B2(TXB2)和一氧化氮(NO)的浓度变化趋势和蹄部的病理变化,探寻蹄叶炎的发病机制,寻找可以作为早期快速诊断的生物指标,并为蹄叶炎综合预防与治疗提供理论基础。将21匹蒙古马随机分为实验组和对照组。实验组马匹灌服10 g/kg体重的OF,对照组马匹灌服蒸馏水。每隔4 h采集血液样本一次,直至72 h。实验使用Obel分级标准对跛行程度进行评估,将一匹跛行达到3级的马进行安乐死,沿蹄部矢状面剖开,去掉第三趾骨,取出蹄叶层做HE染色和PAS染色。实验结果表明,实验组中有11匹马表现出体温升高,呼吸、心率加快、跛行、腹泻等临床症状,实验组出现临床症状的11匹马在灌服OF后8-20 h陆续出现一级跛行,24-56 h陆续出现二级跛行。随着二级跛行的出现,马匹呼吸率和心率增加,粪便p H值在20 h下降到最低值,直肠温度在40 h达到峰值。所有实验组马匹在12-24 h期间都发生腹泻,所有临床症状均在60 h后逐渐减轻。对照组的马均未出现体温升高,呼吸、心率加快、跛行、腹泻等临床症状。X光检查发现实验组马匹第三指骨发生旋转,对照组马匹第三指骨未见旋转。HE及PAS染色发现实验组马匹蹄叶层出现炎性细胞浸润、嗜碱性染料聚集在血管周围、细胞变形等组织学变化,对照组马匹蹄叶层未见组织学变化。实验组表现出现临床症状的11匹马血浆LPS、ADPN、胰岛素、血糖、IL-6、p38MAPK、5-HT、ET-1、TXB2和NO浓度均在不同时段变化显著,对照组马匹血浆浓度无显著变化。LPS浓度从8 h时显著上升,持续到40h,在16 h达到峰值,与0 h相比差异极显著(P0.01)。内分泌因子ADPN和胰岛素分别从4 h、8 h显著上升,在36 h分别达到最低值和峰值,与0 h相比差异极显著(P0.01);血糖从4 h显著升高并达到峰值,与0 h相比差异极显著(P0.01),而后迅速降低,在16h恢复至正常水平。炎性指标IL-6从24 h显著升高,持续至68 h,在32 h达到峰值,与0h相比差异极显著(P0.01)。p38MAPK从8 h显著升高,在16 h达到峰值,与0 h相比差异极显著(P0.01);血管收缩剂5-HT、ET-1、TXB2分别从4 h、8 h、8 h显著上升,持续至60 h左右,分别在24 h、28 h、28 h达到峰值,与0 h相比差异极显著(p0.01);NO浓度在8 h显著上升,持续至60 h,在32 h达到峰值,与0 h相比差异极显著(P0.01)。结果表明在OF蹄叶炎模型中,大量LPS入血导致IL-6、ET-1、ADPN和胰岛素等多种物质分泌紊乱,诱发蹄叶炎;LPS、ADPN、胰岛素、IL-6、5-HT、ET-1、TXB2和NO的浓度变化趋与蹄叶炎病程变化相符。
[Abstract]:The disease of sport system is a kind of disease that does great harm to equine, and phlogistic is the most common disease in the disease of sport system. Based on this, we studied the lipopolysaccharide (LPS), adiponectin (ADPN), insulin, blood glucose in the blood circulation of equine fructooligosaccharide (OF) acute hoof phlogistic model. In order to explore the pathogenesis of hoofleaf disease, the concentration trend and pathological changes of interleukin-6 (IL-6) and p38 mitogen-activated protein kinase (p38 MAPKK, 5-HT, ET-1, TXB2, TXB2) and nitric oxide (no) were studied in order to explore the pathogenesis of hoofleaf disease. It can be used as a biomarker for early and rapid diagnosis and provides a theoretical basis for comprehensive prevention and treatment of hoofnut disease. Twenty-one Mongolian horses were randomly divided into experimental group and control group. The experimental group was given 10 g/kg weight of OF, while the control group was given distilled water. Blood samples were collected every 4 hours until 72 hours. The limping degree was evaluated by Obel grading standard. A horse with grade 3 lameness was euthanized. The third phalanx was cut off along the sagittal plane of the hoof, and the leaf layer of the hoof was stained by HE and PAS staining. The results showed that 11 horses in the experimental group showed clinical symptoms such as elevated body temperature, rapid breathing, rapid heart rate, lameness, diarrhea, etc. In the experimental group, 11 horses with clinical symptoms appeared first grade claudication from 8 to 20 hours after administration of OF and 24 to 56 hours of secondary claudication. With the appearance of secondary lameness, the respiration rate and heart rate of the horse increased, the pH value of feces decreased to the lowest value at 20 h, and the rectal temperature reached the peak at 40 h. All the horses in the experimental group developed diarrhea between 12 and 24 hours, and all the clinical symptoms gradually decreased after 60 hours. The horses in the control group had no symptoms such as elevated body temperature, rapid breathing, rapid heart rate, claudication, diarrhea and other clinical symptoms. X-ray examination showed that the third phalanx bone of the horses in the experimental group rotated. In the control group, the third phalanx of the control group was not rotated. He and PAS staining showed that there were inflammatory cells infiltrating in the leaf layer of the horse's hoof in the experimental group, the basophilic dye gathered around the blood vessel, cell deformation and other histological changes were found, but no histological changes were found in the leaf layer of the horse's hoof in the control group. In the experimental group, the plasma levels of LPS-ADPN, insulin, IL-6 / p38MAPKK _ (5-HTT _ (-1) TXB2 and no were significantly changed in 11 horses with clinical symptoms, while in the control group, there was no significant change in plasma concentration. The concentration of LPS increased significantly from 8 h to 8 h. After 40 h, the peak value was reached at 16 h, and the difference between 0 h and 0 h was significant (P 0.01). The endocrine factor ADPN and insulin increased significantly from 4 h to 8 h, and reached the lowest value and peak value at 36 h, respectively, and the difference was very significant compared with 0 h (P 0.01), and the blood glucose increased significantly from 4 h to the peak. Compared with 0 h, the difference was very significant (P 0.01), then decreased rapidly, and returned to normal level at 16 h. The inflammatory index IL-6 increased significantly from 24 h to 68 h, reached the peak at 32 h, and increased significantly from 8 h to 16 h compared with 0 h. Compared with 0 h, 5-HTET-1 TXB2 increased significantly from 4 h to 8 h, lasted for about 60 h, and reached its peak at 24 h, 28 h and 28 h, respectively. Compared with 0 h, the concentration of no in 5-HTET-1 + TXB2 increased significantly at 8 h. After 60 h, the peak value was reached at 32 h, and the difference between 0 h and 0 h was significant (P 0.01). The results showed that in the model of hoof leaf inflammation, a large amount of LPS induced the disturbance of secretion of IL-6ET-1 ADPN and insulin, and induced the changes of the concentrations of TXB2 and no in LPSN, IL-65-HTET-1, TXB2 and no were consistent with the course of philitis.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.21

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