猪印记基因MKRN3的SNPs筛选及胴体性状关联分析

发布时间：2018-05-23 21:50

本文选题：猪 + MKRN　；参考：《华中农业大学学报》2017年06期

【摘要】：以梅山猪、大白猪和长白猪为研究对象,筛选出MRKN3基因的单核苷酸多态位点(SNPs)并进行PCR-RFLP验证,利用PCR-Bst UI-RFLP对大白×梅山F2代资源家系(285头)DNA样品酶切分型,将分型结果与猪胴体性状进行关联分析。结果表明:猪MRKN3基因编码区有7个SNPs,分别为293CG、361GA、497GA、630GA、678TC、1376CT、和1407TA;其中678TC位点等位基因频率在中外不同猪种中存在差异;猪MRKN3基因678TC位点与内脂率及臀部膘厚显著相关(P0.05);678TC位点对内脂率具有显性效应(P0.05),表现为该位点杂合子比纯合子具有更高的内脂率;而该位点对臀部膘厚具有加性效应(P0.05),表现为等位基因678C的累加具有降低臀部膘厚的效应。因此,猪MKRN3基因的678TC位点可为猪的分子育种实践提供一定指导作用。
[Abstract]:The single nucleotide polymorphisms (SNPs) of MRKN3 gene were screened out from Meishan pig, big white pig and Landrace pig, and PCR-RFLP was used to verify the results. PCR-Bst UI-RFLP was used to type 285 MRKN3 samples from F _ 2 family of Dabai 脳 Meishan F _ 2 generation. The results of typing were correlated with carcass traits of pigs. The results showed that there were 7 SNPs in the coding region of porcine MRKN3 gene, which were 293CG361GA497 GA-630GA-678TCf1376CTT and 1407TA.The frequency of 678TC alleles was different between Chinese and foreign pig breeds. The 678TC locus of porcine MRKN3 gene was significantly correlated with internal fat rate and buttocks fat thickness. P0.05Tc locus had a dominant effect on internal fat rate, which showed that the heterozygote had higher internal fat rate than homozygote. This locus has an additive effect on buttocks fat thickness, which shows that the accumulation of allele 678C has the effect of decreasing the buttocks fat thickness. Therefore, the 678TC locus of porcine MKRN3 gene can provide some guidance for pig molecular breeding practice.
【作者单位】：武汉轻工大学动物营养与饲料科学湖北省重点实验室;华中农业大学农业动物遗传育种与繁殖教育部重点实验室;
【基金】：国家自然科学基金项目(31601922) 湖北省自然科学基金项目(2014CFB345)
【分类号】：S828.2

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