2016年-2017年四川省部分地区猪繁殖与呼吸综合征病毒的分子流行病学调查

发布时间：2018-05-24 07:24

本文选题：猪繁殖与呼吸综合征病毒 + GP　；参考：《动物医学进展》2017年12期

【摘要】：为了解近年来四川省猪繁殖与呼吸综合征病毒(PRRSV)流行毒株的遗传变异和分子流行病学情况,通过RT-PCR方法,对四川省各地疑似病料进行PRRSV检测,确定获得阳性样本基因型和对GP5基因遗传进化进行分析。结果显示,149份疑似病料中经ORF7片段检测33份为阳性,阳性率为22.15%(95%CI:15.8%~29.7%);通过对NSP2片段的检测,其中有9份为PRRSV高致病性毒株(HP-PRRSV),5份为PRRSV经典毒株,8份为PRRSV NADC-30样毒株;通过对GP5基因进行扩增和序列测定,得到11株部分的GP5基因序列数据,所获序列与已报道对应核苷酸序列同源性为60.4%~99.8%,推导的氨基酸序列的同源性为74.3%~100%。结果表明,近两年四川地区PRRSV主要流行毒株为HP-PRRSV,同时新的变异毒株NADC30的流行呈上升趋势。四川地区乃至国内各地区的PRRSV基因在逐渐变异,且毒株的流行趋势在转变,提示应加强对PRRSV流行及变异的监测。
[Abstract]:In order to understand the genetic variation and molecular epidemiology of porcine reproductive and respiratory syndrome virus (PRRSVV) epidemic strains in Sichuan Province in recent years, PRRSV was used to detect suspected samples from different parts of Sichuan province by RT-PCR method. To determine the genotypes of positive samples and to analyze the genetic evolution of GP5 gene. The results showed that 33 of 149 suspected samples were positive by ORF7 fragment, the positive rate was 22.15% and 95% CI: 15.8% and 29.775%, 9 of them were PRRSV high pathogenic virus strain HP-PRRSVV, 5 were PRRSV classical virus strains, 8 were PRRSV NADC-30 strains. By amplifying and sequencing the GP5 gene, the partial GP5 gene sequence data of 11 strains were obtained. The homology of the obtained sequence with the reported nucleotide sequence was 60.4 and 99.8, and the deduced amino acid sequence was 74.3100. The results showed that HP-PRRSVV was the main epidemic strain of PRRSV in Sichuan in recent two years, and the prevalence of new variant strain NADC30 was on the rise. The PRRSV gene is mutating gradually and the epidemic trend of the virus strain is changing in Sichuan area and even in China. It is suggested that the surveillance of PRRSV epidemic and variation should be strengthened.
【作者单位】：西南民族大学生命科学与技术学院;云南农业职业技术学院;国家民族事务委员会青藏高原动物疫病防控创新团队;
【基金】：“十三五”国家重点研发计划项目(2016YFD0500705) 西南民族大学研究生创新科研项目(CX2017SZ052)
【分类号】：S852.651

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