绵羊肺炎支原体免疫组织化学及间接免疫荧光检测方法的建立

发布时间：2018-05-24 23:30

本文选题：绵羊肺炎支原体 + 免疫荧光　；参考：《中国兽医科学》2017年02期

【摘要】：以病理组织学观察为基础,建立检测绵羊肺炎支原体的间接免疫组织化学和间接免疫荧光方法,以绵羊肺炎支原体标准血清为一抗,分别以家兔抗山羊Ig G-HRP和家兔抗山羊Ig G-FITC为二抗,对绵羊肺炎支原体阳性肺组织切片及阴性对照组切片进行HE染色、免疫组织化学染色及间接免疫荧光染色,并优化染色条件,以确保组织切片在最佳的条件下进行鉴定。结果显示,本试验所建立的免疫组织化学方法与PCR方法对病样的检出率相符,且高于传统的分离培养法。结果表明,本试验建立的间接免疫组织化学方法和间接免疫荧光方法可用于对临床病例的组织样本检测,具有很强的特异性和可靠性,可直观地观察和探究该病原在机体内的定位、动态分布以及致病机理,以期为临床诊断提供更可靠的方法。
[Abstract]:On the basis of histopathological observation, an indirect immunohistochemical and indirect immunofluorescence method for the detection of Mycoplasma pneumoniae in sheep was established. The standard serum of Mycoplasma pneumoniae was used as the first antibody. Using rabbit anti-goat Ig G-HRP and rabbit anti-goat Ig G-FITC as second antibodies, HE staining, immunohistochemical staining and indirect immunofluorescence staining were performed on the positive lung tissue sections of sheep mycoplasma pneumoniae and negative control groups, and the staining conditions were optimized. To ensure that tissue sections are identified under optimal conditions. The results showed that the immunocytochemical method established in this study was consistent with the detection rate of PCR method and was higher than that of the traditional isolation culture method. The results show that the indirect immunohistochemical method and indirect immunofluorescence method established in this study can be used to detect the tissue samples of clinical cases, and have strong specificity and reliability. The location, dynamic distribution and pathogenesis of the pathogen in the organism can be observed and explored intuitively in order to provide a more reliable method for clinical diagnosis.
【作者单位】：内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室内蒙古预防重点实验室;
【基金】：国家科技支撑计划项目(2012BAD13B00) 内蒙古自治区科技计划项目(201502070)
【分类号】：S858.26

【相似文献】