鸭特异性CpG ODN体外抑制鸭坦布苏病毒复制的研究

发布时间：2018-05-26 16:39

本文选题：鸭 + CpG　；参考：《中国家禽》2017年16期

【摘要】：为明确鸭特异性寡聚脱氧核苷酸(Cp G ODN)体外对鸭坦布苏病毒(DTMUV)复制的抑制作用,研究在Cp G ODN最佳活性分子筛选的基础上,与DTMUV感染后的细胞进行共孵育,分别采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法(MTT法)和实时荧光定量PCR(q RT-PCR)检测不同浓度Cp G ODN-D2对DTMUV复制的抑制作用。结果显示:对非洲绿猴肾(Vero)细胞来说,Cp G ODN-D2浓度在300μg/m L以内均为安全浓度;MTT法和q RT-PCR检测结果均表明,各浓度Cp G ODN-D2对DTMUV的复制均有一定的抑制作用,并随浓度升高呈现先上升后下降的趋势,其中30μg/m L的浓度效果最佳,达到84.74%。结果证实了Cp G ODN-D2作为新型免疫调节剂可有效抑制DTMUV的复制。
[Abstract]:In order to determine the inhibitory effect of duck specific oligodeoxynucleotides (CPG ODN) on the replication of duck tambusuvirus (DTMUV) in vitro, we studied the co-incubation with DTMUV infected cells on the basis of screening the best active molecules of DTMUV. The inhibitory effects of different concentrations of CP G ODN-D2 on DTMUV replication were detected by using 3-trimethythiazole-2-dimethylthiazolium 5-diphenyl tetrazolium bromide method and real-time fluorescence quantitative PCR(q RT-PCR, respectively. The results showed that the concentration of CpG ODN-D2 was safe within 300 渭 g / mL and the results of Q RT-PCR assay showed that CpG ODN-D2 could inhibit the replication of DTMUV to some extent. The concentration of 30 渭 g / mL was the best, reaching 84.74. The results showed that CP G ODN-D2 could effectively inhibit the replication of DTMUV as a new immunomodulator.
【作者单位】：山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室;河北工程大学生命科学与食品工程学院;
【基金】：“十三五”国家重点研发计划课题(2016YFD0500106) “十二五”农村领域国家科技计划项目(2015BAD12B03) 山东省农业科学院农业科技创新工程(CXGC2016B14) 泰山学者特聘专家工程经费(ts201511069)
【分类号】：S852.65

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