香猪FSHR基因外显子10的多态性及其与产仔数间的关联分析

发布时间：2018-05-27 04:34

本文选题：FSHR基因 + 多态性　；参考：《中国畜牧兽医》2017年03期

【摘要】：促卵泡激素(follicle-stimulating hormone,FSH)是垂体前叶分泌的一种糖蛋白,可通过卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)调节卵巢的功能。为了研究香猪FSHR基因外显子10的多态性及其与产仔数的关系,试验以从江香猪为研究对象,采用PCR产物直接测序方法检测基因的多态性,分析基因型与香猪产仔数间的关系。对8个候选位点进行群体检测证实其中2个为SNPs位点,分别是rs322800083(C276T)和rs332115220(T601C),且新发现1个SNP位点novel 1(G-92A);rs322800083和rs332115220位点构成4种单倍型,其中C-T为香猪群体的主要单倍型(45.0%),且在香猪群体中紧密连锁(r2=0.334)。分析4种单倍型与香猪产仔数间的关系,在香猪的二胎产仔数中,单倍型C-T的总产仔数(total number born,TNB)最高,单倍型C-C的TNB最低,两者之间的TNB相差0.99头(P0.05);在香猪的三胎产仔数中,单倍型C-T的TNB最高,与单倍型T-C、C-C间的TNB分别相差1.61和2.33头(P0.01)。rs332115220位点的基因型与香猪的三胎产仔数呈弱的正相关关系(ρ=0.429)。本研究结果提示,单倍型C-T可以作为分子标记应用于香猪产仔数性状的辅助选育。
[Abstract]:Follicle-stimulating hormone (FSH) is a glycoprotein secreted by the anterior pituitary gland, which regulates ovarian function through follicle-stimulating hormone receptor (FSHR). In order to study the polymorphism of exon 10 of FSHR gene in Xiang pig and its relationship with litter size, the polymorphism of gene was detected by direct sequencing of PCR products in Congjiang Xiang pig, and the relationship between genotype and litter size was analyzed. Two of the 8 candidate loci were identified as SNPs loci, rs322800083 (C276T) and rs332115220 (T601CU). One SNP locus, novel 1G -92A, rs322800083, and rs332115220 locus were found to form four haplotypes. C-T was the main haplotype of Xiang pig population. By analyzing the relationship between four haplotypes and piglets, the total litter size of haplotype C-T was the highest, and the TNB of haplotype C-C was the lowest, the difference of TNB between them was 0.99 and 0.99, respectively, and in the third litter size of Xiang pig, the total litter size of C-T was the highest and the TNB of haplotype C-C was the lowest. The TNB of haplotype C-T was the highest, and the TNB of haplotype C-T was 1.61 and 2.33, respectively. There was a weak positive correlation between the genotype of P0.01U. Rs332115220 and the third litter size of Xiang pig. The results suggested that haplotype C-T could be used as a molecular marker in assisted breeding of piglets litter size traits.
【作者单位】：贵州大学生命科学学院;贵州大学农业生物工程研究院;贵州大学动物科学学院;铜仁学院;
【基金】：国家高技术研究发展计划(863计划)课题(2013AA102503) 贵州省农业攻关项目(黔科合NY[2013]3073号) 贵州省"百"层次创新型人才项目(黔科合人才2016-4012号) 国家自然科学基金(31672390)
【分类号】：S828

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