谷氨酰胺对脂多糖诱导的断奶仔猪氧化应激的影响

发布时间：2018-05-27 20:56

本文选题：谷氨酰胺 + 断奶仔猪　；参考：《动物营养学报》2017年04期

【摘要】：本试验以大肠杆菌型脂多糖(LPS)建立氧化应激模型,探讨了谷氨酰胺(GLN)对断奶仔猪氧化应激的影响。选用24头28日龄的健康三元(杜×长×大)断奶仔猪,随机分成3组,每组8个重复,每个重复1头猪。对照组和应激组饲喂基础饲粮,GLN组饲粮在基础饲粮中添加1%GLN,试验期为30 d。在试验第22、25、28和30天,应激组和GLN组仔猪分别按每千克体重腹腔注射100μg LPS,对照组仔猪腹腔注射相同剂量的灭菌生理盐水,第30天进行前腔静脉采血并屠宰采取所需肠道样品,检测氧化应激相关指标。结果显示:1)LPS攻毒前各组血清抗氧化能力指标均无显著差异(P0.05)。LPS攻毒后,应激组血清丙二醛(MDA)含量显著高于对照组(P0.05);GLN组血清MDA含量和超氧化物歧化酶(SOD)活性显著低于应激组和对照组(P0.05)。2)LPS攻毒后,在十二指肠黏膜中,GLN组过氧化氢酶(CAT)和锌铜超氧化物歧化酶(Cu Zu SOD)基因相对表达量显著高于应激组(P0.05)。在空肠黏膜中,GLN组CAT、锰超氧化物歧化酶(Mn SOD)、谷胱甘肽过氧化物酶1(GPX1)和谷胱甘肽过氧化物酶4(GPX4)基因相对表达量显著高于对照组和应激组(P0.05),对照组GPX4基因相对表达量显著高于应激组(P0.05)。在回肠黏膜中,GLN组和应激组CAT基因相对表达量显著低于对照组(P0.05),GPX4基因相对表达量显著高于对照组(P0.05);GLN组Mn SOD基因相对表达量显著高于对照组和应激组(P0.05),Cu Zn SOD基因相对表达量显著高于对照组(P0.05)。结果表明,GLN在一定程度上可以缓解断奶仔猪因LPS引起的氧化应激,以期为实际生产中减少氧化应激提供一定的理论基础。
[Abstract]:The effect of GLN on oxidative stress in weaned piglets was studied by using LPS-based lipopolysaccharide (LPS) of E. coli to establish oxidative stress model. Twenty-four 28 day-old healthy piglets were randomly divided into 3 groups, 8 replicates in each group, and 1 pig per replica. The control group and the stress group were fed with GLN in the basal diet for 30 days. On the 28th and 30th day of the experiment, the piglets in stress group and GLN group were injected 100 渭 g LPSper kg BW intraperitoneally respectively, while the control group was injected with the same dose of sterilized saline intraperitoneally. On the 30th day, the blood was collected from the anterior vena cava and slaughtered to take the intestinal sample. The related indexes of oxidative stress were detected. The results showed that there was no significant difference in the indexes of serum antioxidant capacity between the groups before and after LPS attack, and there was no significant difference between the two groups before and after LPS attack. The content of serum malondialdehyde (MDA) in the stress group was significantly higher than that in the control group (P 0.05) and the activity of superoxide dismutase (SOD) was significantly lower than that in the stress group and the control group. In duodenal mucosa, the relative expression of catalase (CAT) and zinc copper superoxide dismutase (Cu Zu SOD) gene in GLN group was significantly higher than that in stress group (P 0.05). The relative expression of GPX4 gene in GLN group was significantly higher than that in control group and stress group, and the relative expression of GPX4 gene in control group was significantly higher than that in control group and stress group (P 0.05), and the relative expression of Glutathione peroxidase (Glutathione peroxidase 1) and glutathione peroxidase (Glutathione peroxidase 4GPX4) in Glutathione peroxidase group was significantly higher than that in control group and stress group. It was significantly higher than that of stress group (P 0.05). In ileal mucosa, the relative expression of CAT gene in GLN group and stress group was significantly lower than that in control group (P 0.05). The relative expression of mn SOD gene in control group was significantly higher than that in control group and stress group. The relative expression of mn SOD gene was significantly higher than that in control group and stress group. The expression of P0.05 in the control group was significantly higher than that in the control group. The results showed that GLN could alleviate oxidative stress caused by LPS in weaned piglets to a certain extent, and provide a theoretical basis for reducing oxidative stress in practical production.
【作者单位】：湖南农业大学动物科技学院;中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术中心农业部中南动物营养与饲料科学观测实验站;中国科学院大学;
【基金】：国家973项目专题(2013CB127301) 中科院“百人计划”项目国家自然科学基金面上项目(31472107)
【分类号】：S828.5

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