日本血吸虫小RNA let-7对虫体生殖发育影响的研究

发布时间：2018-05-28 03:11

本文选题：日本血吸虫 + Sja-let-　；参考：《中国预防兽医学报》2017年05期

【摘要】：为检测日本血吸虫小RNA let-7(Sja-let-7)对虫体生殖发育的影响,本研究在日本血吸虫尾蚴感染BALB/c小鼠后的11 d~42 d,将Sja-let-7的同系物(Sja-let-7 mimics)注射入宿主体内对血吸虫Sja-let-7的表达进行干扰,结果显示Sja-let-7 mimics干扰组小鼠肝脏虫卵孵化率显著升高;小鼠肝脏肉芽肿病变减轻十分明显,病灶周围炎性细胞浸润半径变小,空泡变性较对照组轻微,周围正常组织完整性也优于对照组。扫描电镜和透射电镜未观察到虫体的明显变化。利用荧光定量PCR检测Sja-let-7的候选靶基因干扰虫体的表达情况,结果表明Sja-let-7 mimics对大部分候选基因存在不同程度的抑制作用。本研究为后续深入研究小RNA Sja-let-7的功能奠定了基础。
[Abstract]:In order to detect the effect of small RNA let-7 Sja-let-7) on the reproductive development of Schistosoma japonicum, the Sja-let-7 congener Sja-let-7 mimicswas injected into the host to interfere with the expression of Sja-let-7 in mice infected with Schistosoma japonicum cercariae at 11 days and 42 days after infection. The results showed that the hatching rate of liver eggs in Sja-let-7 mimics interference group was significantly higher than that in control group, the pathological changes of liver granuloma in mice were obviously alleviated, the infiltration radius of inflammatory cells around the lesion was smaller, and the vacuolar degeneration was less than that in control group. The peripheral normal tissue integrity was also superior to that of the control group. No obvious changes were observed under scanning electron microscope and transmission electron microscope. Fluorescence quantitative PCR was used to detect the expression of candidate target genes in Sja-let-7. The results showed that Sja-let-7 mimics had different inhibitory effects on most candidate genes. This study lays a foundation for further research on the function of small RNA Sja-let-7.
【作者单位】：中国农业科学院上海兽医研究所国家防治动物血吸虫病专业实验室/农业部动物寄生虫学重点实验室;
【基金】：家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2015KFKT0014) 上海市自然科学基金(16ZR1444100) 国家自然科学基金(31672245)
【分类号】：S852.735

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