BMP15基因上1个新的影响绵羊繁殖力的SNP位点

发布时间：2018-05-28 12:49

本文选题：骨骼形态发育基因(BMP) + 阿勒泰羊　；参考：《西南农业学报》2017年09期

【摘要】：【目的】阿勒泰羊是新疆北部地区重要的产多羔绵羊品种,但一直未找到影响其高繁殖力的主效基因。以影响排卵数的BMP15基因作为候选基因,从分子水平上对阿勒泰的多胎机制进行研究。【方法】以PCR直接测序法获得SNP位点,分析突变位点的特性,并通过大规模的群体检测统计推断其遗传效应。【结果】阿勒泰羊在BMP15基因的内含子667 bp和外显子2的755 bp处发生突变,2个位点均只有2种基因型,且纯合子为优势基因。与产羔数关联分析发现仅外显子2的755 bp处位点2种基因型差异显著,经群体验证得到同样结果,TC型母羊比TT型母羊平均多产0.8羔。同时该处的突变引起了氨基酸由亮氨酸向脯氨酸变化。【结论】故推测BMP15基因与控制阿勒泰羊多胎性能的主效基因存在紧密的遗传连锁。
[Abstract]:Objective: Altay sheep is an important sheep breed in northern Xinjiang, but no major gene has been found to affect its high fecundity. BMP15 gene, which affects ovulation number, was used as candidate gene to study the multifetal mechanism of Altay at molecular level. [methods] the SNP loci were obtained by PCR direct sequencing, and the characteristics of mutation sites were analyzed. The genetic effect of Altay sheep was deduced by large scale population analysis. [results] there were mutations at 667bp in intron of BMP15 gene and 755bp in exon 2. There were only two genotypes at the two loci, and homozygous gene was the dominant gene. Only 755 BP loci of exon 2 showed significant difference between the two genotypes. The same result was obtained by population verification. The average yield of TC type ewe was 0.8 more than that of TT ewe. At the same time, the mutation caused the change of amino acid from leucine to proline. [conclusion] it is concluded that there is a close genetic linkage between the BMP15 gene and the main gene that controls the fertility of Altay sheep.
【作者单位】：石河子大学动物科技学院;农业部草食家畜遗传育种与繁殖重点开放实验室新疆维吾尔自治区畜牧科学院生物技术研究中心;新疆维吾尔自治区阿勒泰地区畜牧兽医工作站;
【基金】：中国科学院“西部青年学者”项目(2015-XBQN-A-08)
【分类号】：S826

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