扬州及周边地区动物源性沙门菌的流行特征及外排泵分析

发布时间：2018-05-28 14:16

本文选题：沙门菌 + 扬州及周边地区　；参考：《扬州大学》2017年硕士论文

【摘要】：沙门菌是一种重要的人畜共患病病原,它不仅对畜禽养殖业带来了巨大的经济损失,而且具有重要的公共卫生学意义。沙门菌的血清型目前有2600多种,且大多数对人类有一定致病性,是引起食物中毒的主要病原之一。沙门菌病是由沙门菌属细菌引起的各种动物和人疾病的总称,临床上主要表现为败血症和肠炎等。本研究对采集自扬州大学动物医院的动物病料样品进行了沙门菌的分离、鉴定以及药敏实验;运用PCR技术检测并分析了沙门菌外排泵的相关情况。本研究的目的在于对本地区流行的沙门菌进行调查并为沙门菌外排泵的相关研究提供理论依据。一、沙门菌的分离鉴定与血清型分析本文研究的目的之一在于调查扬州及周边地区沙门菌的流行情况。2015年7月到2017年2月,从扬州大学动物医院采集病死动物病料860份,经过对病料进行预增菌、增菌、纯化和PCR鉴定,共分离出126株沙门菌,分离率为14.65%。血清型鉴定结果表明分离株当中的鼠伤寒血清型占所有分离株的45.23%,其次为肠炎沙门菌,占比17.91%,说明扬州及周边地区鼠伤寒沙门菌为优势血清型。二、沙门菌的耐药性分析运用药敏纸片扩散法对分离到的126株沙门菌进行药敏实验。结果显示69.0%的分离株对多西环素的耐药,42.1%的分离株对链霉素耐药。同时沙门菌的多重耐药性也十分严重,在126株沙门菌分离株中,有48株沙门菌耐药3种以上受试抗生素,多重耐药性达38.0%。三、沙门菌外排泵分析本文对6种沙门菌外排泵基因emrE、emrB、bcr、mdfA、STY0901、mdtK的检测,通过PCR检测发现各检测基因阳性率均在90%左右,这说明外排泵在沙门菌当中有着广泛的分布。有32株沙门菌分离株对所有受试抗生素均敏感,外排泵基因阳性率也均在90%以上,这说明沙门菌的耐药与否与外排泵是否存在必然联系有待进一步研究。
[Abstract]:Salmonella is an important zoonotic pathogen, which not only brings great economic loss to livestock and poultry breeding, but also has important public health significance. There are more than 2600 serotypes of Salmonella and most of them are pathogenicity to humans and are one of the main pathogens of food poisoning. Salmonella is a general term for various animal and human diseases caused by Salmonella bacteria. The clinical manifestations of Salmonella are septicemia and enteritis. In this study, the samples of animal diseases collected from Yangzhou University Animal Hospital were isolated, identified and drug sensitive tests were carried out, and the related situation of salmonella efflux pump was detected and analyzed by PCR technique. The purpose of this study is to investigate the prevalent salmonella in this area and to provide theoretical basis for the study of salmonella efflux pump. One of the purposes of this study is to investigate the prevalence of salmonella in Yangzhou and its surrounding areas. From July 2015 to February 2017, 860 samples were collected from Yangzhou University Animal Hospital. A total of 126 strains of Salmonella were isolated by pre-inoculation, purification and PCR identification, and the isolation rate was 14.65%. The results of serotype identification showed that the serotype of mouse typhoid was 45.23% of all isolates, followed by Salmonella enteritidis (17.91%), indicating that Salmonella typhimurium was the dominant serotype in Yangzhou and its surrounding areas. Secondly, drug resistance analysis of Salmonella was carried out on 126 Salmonella isolates by using drug sensitive disk diffusion method. The results showed that 69.0% of the isolates were resistant to doxycycline and 42.1% were resistant to streptomycin. At the same time, the multidrug resistance of Salmonella was also very serious. Of 126 Salmonella isolates, 48 were resistant to more than 3 kinds of antibiotics, and the multidrug resistance reached 38.0%. Third, the analysis of Salmonella efflux pump gene emrErBbcrmdfAnSTY0901mdtK was carried out in this paper. The positive rate of each detected gene was about 90% by PCR, which indicated that the efflux pump was widely distributed in Salmonella. 32 isolates of Salmonella were sensitive to all the tested antibiotics, and the positive rate of efflux pump gene was above 90%, which indicated that there was an inevitable relationship between drug resistance and efflux pump of Salmonella.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S852.61

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